Cells from a human prostatic adenocarcinoma cell line (PC3) were maintained in M199 medium, with and without 5% Bobby calf serum, to examine aspects of cellular zinc metab olism. Cultures contained 0.5 to 1.0 x 106 cells/dish. En hanced 65Zn accumulation in response to dexamethasone was observed after 24 hr in culture. The rate of efflux of accumu lated 65Zn was not influenced by this hormone. ...

Sodium butyrate select ively Induces accumulation of metalIoth1onein-I (MT-I) RNA in H4IIE rat hepatoma ce l l s . The Induction 1s rap id; s igni f icant elevation 1n cytoplasnric MT-I RNA can be observed within three hours af ter exposure to 5 mM butyrate. Maximal levels of MT-I RNA are obtained af ter eight hours. Butyrate stimulates MT RNA accumulation 1n the absence of de_ novo protein syn...

Metallothionein (MT) is a metal-binding protein rapidly accreted in many tissues in response to trace elements or hormones. To gain an understanding of the regulation of MT accretion, rates of MT synthesis and degradation were determined by using a decay-kinetics technique. A chicken macrophage-cell line (HD11) that rapidly accretes incremental amounts of MT when stimulated with increasing conc...

for half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. for this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from...

Plants are generally subjected to a combination of different conditions such as magnetic field and soil bacteria in their life. The present investigation tried to compare the effects and interactions of magnetic field and rhizobial inoculant in alfalfa. A pot experiment was performed under a natural condition by a factorial design to ...

background: cellulytic enzymes including carboxymethyl cellulases play the key role in hydrolysis of cellulose, a huge organic carbon reservoir on earth, into monomeric sugars and their eventual conversion into valuable chemicals and energy sources. objectives: in this study, we described the identification of a basidiomycete isolate ndvn01 and optimization of culture conditions and medium comp...

development of modern economy without private sector and high vigilance entrepreneurs is vital to identify opportunities and use of opportunities around the world. in this regard, identifying the influencing factors on emergence and development of entrepreneurship culture can be effective to promote entrepreneurship in our country. for this purpose, the main issue in this study is to identify t...

We developed a multiplex tandem PCR (MT-PCR) assay for the rapid identification of 16 fungi directly from culture. MT-PCR results were concordant with phenotypic identification for all cultures studied (n = 183). The colony MT-PCR assay was rapid (<2 h), sensitive, and specific in identifying fungal pathogens directly from primary isolation plates.