Introduction:  This study sought to assess the cytotoxic effects of nanoparticulate and microparticulate calcium sodium phosphosilicate mouthwashes on human gingival fibroblasts (HGFs). Methods: This in vitro study was conducted on HGFs isolated and cultured in a 48-well plate containing standard culture medium for evaluation of four concentrations of the two m...

background: the development of injectable sustained-release products are of great interest to veterinary pharmaceuticals and animal health business. recently, great attention has been paid to in situ gel-forming chitosan/beta-glycerophosphate (chitosan/β-gp) solutions due to their good biodegradability and thermosensitivity. objectives: the general aim of this study was to prepare a novel in si...

There is a discrepancy in telomere length as measured by signal intensity of telomere restriction fragments on gels and fluorescence in situ hybridization analysis. This difference has been ascribed to the X-region, a segment of subtelomeric DNA that is resistant to being cut by restriction enzymes. To explore the nature of this region, we analyzed the digestibility of an artificial seeded telo...

Background: Herpes simplex virus type-1 (HSV-1) establishes a lifelong latent infection in neurons following primary infection. The existence of latent HSV-1 DNA in the trigeminal ganglia of infected BALB/c mice was examined using a direct in situ PCR technique, based on Digoxigenin-11-dUTP detection system with anti-digoxigenin-peroxidase and 3,3'-diaminobenzidine (DAB) substrate. Methods: Eig...