The first identification of a sterol in Chlorella was by Klosty and Bergmann (5) in 1952 when they isolated ergosterol from Chlorella pyrenoidosa. More recently, Otsuka (6) identified ergosterol and a A5 sterol in Chlorella ellipsoidea. Ergosterol was the principal component of the mixture. Recent work in this laboratory has been centered about the identification of sterols from other species o...

P-Diethylaminoethyl diphenylpropylacetate hydrochloride (SKF 525-A) has been shown to inhibit a number of reactions involved in drug metabolism (1)) including side-chain oxidation, dealkylation, deamination, hydrosylation, and deesterification. More recently this compound was found to decrease significantly the plasma cholesterol levels in several animal species (2). However, these studies affo...

Yields of (14)CO(2) from [2-(14)C]mevalonate and [5-(14)C]mevalonate have been used by others to estimate the activity of the non-sterol-forming pathway, also called the mevalonate shunt pathway, and yields of (14)C in sterols have been used to estimate the activity of the sterol-forming pathway. Both these pathways operate following the conversion of carbon 1 of mevalonate to CO(2). The estima...

The end-product of epidermal differentiation is a stratified layer of corneocytes whose extracellular lipid bilayers provide a permeability barrier. It is generally accepted that the epidermis synthesizes most if not all of the lipids found in this tissue and that extra-epidermal tissues contribute very little to this lipid content. Moreover, the individual epidermal strata in which epidermal l...

introduction: in the present research, the effect of morphine consumption during pregnancy on the development of the embryo’s spinal cord was studied in wistar rat. methods: female wistar rats (wt: 250-300 g) were mated with males. the test group received morphine (0.01 mg/ml) in their drinking water. pregnant rats were later killed with chloroform on the 12th, 13th and 14th days of pregnancy, ...

(pH 7.0) in the following medium: 5% nhexadecane (Tokyo Kasei Co., Tokyo, Japan), 0.1% KH2PO4, 0.4% (NH4)2S04, 0.05% FeSO4 7Hq0, 0.05% MgS04-7H20, and 0.4% yeast extract (Difco). A total of 10.4 g of dry cells was obtained from 2 liters of culture broth in 2 days at 30 C. The CoQ fraction was extracted as described by Lavate et al. (3). A 1-ml amount of the nonsaponifiable extract was taken up ...

Freshly isolated Morris Hepatoma 7777 cells were prepared for a study of the utilization of palmitate and 0-hydroxybulyrale as metabolic fuels compared to other major energy substrates (glucose and glutamine). These cells were capable of oxidizing both |i/-'4C]palmilale and 0[3-'4C]hydroxybutyrate although the rates accounted for only 10 ±3 (SD) and 9 ±4% of total oxygen consumed, respectivel...

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