Control of nuclear RNA stability is essential for proper gene expression, but the mechanisms governing RNA degradation in mammalian nuclei are poorly defined. In this study, we uncover a mammalian RNA decay pathway that depends on the nuclear poly(A)-binding protein (PABPN1), the poly(A) polymerases (PAPs), PAPα and PAPγ, and the exosome subunits RRP6 and DIS3. Using a targeted knockdown approa...

Translation arrest leads to an endonucleolytic cleavage of mRNA that is termed no-go decay (NGD). It has been reported that the Dom34:Hbs1 complex stimulates this endonucleolytic cleavage of mRNA induced by translation arrest in vivo and dissociates subunits of a stalled ribosome in vitro. Here we report that Dom34:Hbs1 dissociates the subunits of a ribosome that is stalled at the 3' end of mRN...

A possible model independent test of the theoretically calculated nuclear matrix elements of 0νββ-decay is proposed. The test can be accomplished if 0νββ-decay of three (or more) nuclei is observed. The selection of the nuclei for the next generation of 0νββ-decay experiments should be done taking into account also considerations regarding the possibility to test the nuclear matrix element calc...

The human nuclear poly(A)-binding protein PABPN1 has been implicated in the decay of nuclear noncoding RNAs (ncRNAs). In addition, PABPN1 promotes hyperadenylation by stimulating poly(A)-polymerases (PAPα/γ), but this activity has not previously been linked to the decay of endogenous transcripts. Moreover, the mechanisms underlying target specificity have remained elusive. Here, we inactivated ...

A possible model independent test of the theoretically calculated nuclear matrix elements of 0νββ-decay is proposed. The test can be accomplished if 0νββ-decay of three (or more) nuclei is observed. The selection of the nuclei for the next generation of 0νββ-decay experiments should be done taking into account also considerations regarding the possibility to test the nuclear matrix element calc...

A Co-ordinated Research Project (CRP) was established in 1998 by the IAEA Nuclear Data Section (Update of X-ray and gamma-ray Decay Data Standards for Detector Calibration and Other Applications), in order to improve further the recommended decay data used to undertake efficiency calibrations of gamma-ray detectors. Participants in this CRP reviewed and modified the list of radionuclides most s...

The evaluation of the nuclear matrix elements (NME) of the twoneutrino double beta (2νββ) decay and neutrinoless double beta (0νββ) decay using the proton-neutron quasiparticle random-phase approximation (pnQRPA) is addressed. In particular, the extraction of a proper value of the proton-neutron particle-particle interaction parameter, gpp, of this theory is analyzed in detail. Evidence is show...