Colorectal cancer remains one of the major cancer- related deaths despite progress in the treatment during past decades. Detection of disease at earlier stages reduces its mortality. The aim of current study was to investigate expression of Cytokeratin 19 (CK19), Cytokeratin 20 (CK20) and Guanylyl Cyclase C (GCC) mRNA in peripheral blood of non- metastatic colorectal cancer patients which may r...

In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the related cDNA was inserted in two expression plasmids carrying pelB signal peptide, one with lac bacterial promoter and the other with a bacteriophage T7-based promoter. The recombinant plasmids were moved to TG1 and BL21 strains of E. coli, respectively. To induce the expression systems, IPTG and...

background and objectives: adenoid cystic carcinoma (acc), the most common malignant tumor of submandibular and minor salivary glands, is characterized by a high rate of local recurrence and distant hematogenous metastasis and tendency to invade peripheral nerves. in spite of radiotherapy and surgical treatment, the long-term prognosis is dismal. today, many studies are being conducted using th...

the widespread use of sulfur mustard  (sm) as a chemical warfare agent in the  past century has proved its long-lasting toxic effects. despite a lot of research over the past decades on iranian veterans, there are still major gaps in the sm literature. transforming growth  factor  (tgf-β),  a  cytokine  that  affects  many  different  cell processes,  has  an important role in the lungs of pati...

micrornas (mirnas) are a class of small non coding regulatory rnas that have key functions in multiple cell processes. deregulation of these tiny mirnas are involved in various human diseases. mir-155 is one of the multifunctional mirna that its over-expression has been found to be associated with different kinds of cancer such as leukemia, breast and colon cancers. it is thought that deregulat...

background and objective: esophageal cancer especially squamous cell carcinoma (scc) is one of the most common gastro intestinal malignancies in north part of iran (khorasan). the standard treatment for esophageal cancer is surgical resection, but its outcome remains poor. then, the oncologists try to treat this cancer with sandwich protocols especially neo-adjuvant chemo-radiotherapy. several ...

background and objective: p53 is a suppressive gene that plays a key role in dna repair and apoptosis. the purpose of this study was to investigate the effect of p53 protein over-expression and some clinicopathological factors on the esophageal squamous cell carcinoma (scc) response to neoadjuvant chemoradiotherapy. patients and methods: in this retrospective cohort study, 44 patients with loca...

A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...