Capillary columns packed electrokinetically with 1.5-μm nonporous octadecylsilica particles are used to achieve rapid separations with high efficiencies. A mixture containing five polycyclic aromatic hydrocarbons (PAHs) as test compounds is separated in less than 5 s with 28 kV applied to a column with a packed length of 6.5 cm (10 cm total length). A sample containing 16 PAHs (classified as pr...

The sorption of thorium and uranium ions by functionalized SBA–15 mesoporous silica materials with Schiff base ligating groups N–propylsalicylaldimine (SBA/SA) and ethylenediaminepropylesalicylaldimine (SBA/EnSA) from aqueous solution was investigated in fixed-bed column method. The TEMPeffect of pH, sample solution volume, and the column design parameters such as sample and eluent flow rat...

The toxicity and corrosion potential of hydrogen sulfide in raw biogas underlines the need for biogas purification. Several techniques available for removal of hydrogen sulfide from biogas are out of the reach for common end users due to lack of knowledge, higher running costs, and insufficient operational skills. The present experimental study aims to propagate hydrogen sulfide removal techniq...

A flow injection (FI)-reduced volume column system was developed for hemoglobin (Hb) typing to be used as an initial screening method for thalassemia. The column was packed with 140 microl diethylaminoethyl (DEAE)-Sephadex A-50 ion exchange beads. Hb can be separated using Tris-HCl buffer solution with pH gradient 8.5-6.5 and then monitored spectrophotometrically at 415 nm. The hemolysate of 40...

Duplex capillary columns, the standard for electrochromatography using optical detection, consist of a packed and an open section. Normally, optical detection is performed in an on-column manner, i.e. at a point right after the packed section. It was deemed that band broadening may take place when an analyte band travels from the packed bed, through the frit and down to the open section. In thi...

Physical characteristics critical to chromatography including geometric porosity and tortuosity within the packed column were analysed based upon three dimensional reconstructions of bed structure in-situ. Image acquisition was performed using two X-ray computed tomography systems, with optimisation of column imaging performed for each sample in order to produce three dimensional representation...

Immune complexome analysis is a method for identifying and profiling of antigens in circulating immune complexes (CICs); it involves separation of immune complexes from serum, direct tryptic digestion of these complexes, and protein analysis via nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS). To improve this method, we initially investigated the effects of two factors-the g...