The local synthesis of dopamine and its effects on insulin release have been described in isolated islets. Thus, it may be accepted that dopamine exerts an auto-paracrine regulation of insulin secretion from pancreatic beta cells. The aim of the present study is to analyze whether dopamine is a regulator of the proliferation and apoptosis of rat pancreatic beta cells after glucose-stimulated in...

Human pluripotent stem cell (hPSCs) derived-pancreatic islets (hSC-islets) are good candidates for replacement therapy patients with diabetes as substitutes deceased donor-derived islets, because they and have infinite proliferation potential. Grafted hSC-islets ameliorate hyperglycemia in diabetic mice; however, several weeks needed to normalize the hyperglycemia. These data suggest require ma...

Vanadium salts have been suggested as a possible therapeutic agent for the treatment of diabetes. The aim of the present study was to clarify histological and immunohistochemical changes that occur in the pancreatic beta (β) cells of vanadyl sulphate (VS)-treated streptozotocin (STZ) induced diabetic rats. Male Wi star rats were made diabetic by injecting a single intravenous dose of STZ (...

vanadium salts have been suggested as a possible therapeutic agent for the treatment of diabetes. the aim of the present study was to clarify histological and immunohistochemical changes that occur in the pancreatic beta (β) cells of vanadyl sulphate (vs)-treated streptozotocin (stz) induced diabetic rats. male wi star rats were made diabetic by injecting a single intravenous dose of stz (40 mg...

Approximately half of the transplantable pancreatic islet tissue is lost during isolation, including the digestion and purification steps. Modifying the isolation method could increase the yield. This would enable the one donor-one recipient concept and improve the therapeutic effects of islet transplantation. This study aims to improve islet transplantation by increasing the yield of islets fr...

The NOD mouse develops spontaneous type 1 diabetes, with some features of disease that are very similar to the human disease. However, a proportion mice naturally protected from developing and currently, studies characterizing this cohort limited. Here, using both immunofluorescence multiparameter flow cytometry, we focus on pancreatic islet morphology immune infiltrate observed in mice. We sho...

The endocrine portion of the pancreas, which is characterized by pancreatic islets, has been widely investigated among different species. The BALB/c and C57BL/6 mice are extensively used in experimental research, and the morphometric differences in the pancreatic islets of these animals have not been evaluated so far. Thus, our data have a comparative perspective related to the morphometric ana...

PURPOSE Hyperglycemia and abnormal glucose tolerance tests observed in some patients with chronic Chagas' disease suggest the possibility of morphological changes in pancreatic islets and/or denervation. The purpose of this study was to describe the morphology and morphometry of pancreatic islets in chronic Chagas' disease. METHODS Morphologic and computerized morphometric studies were perfor...

The clinical usability of pancreatic islet transplantation for the treatment of type I diabetes, despite some encouraging results, is currently hampered by the short lifespan of the transplanted tissue. In vivo studies have demonstrated that co-transplantation of Mesenchymal Stem Cells (MSCs) with transplanted pancreatic islets is more effective with respect to pancreatic islets alone in ensuri...

The present study was conducted to clarify the regional distribution and relative frequency of endocrine cells secreting insulin, glucagon, orexin A (OXA) and ghrelin in the pancreatic islets of guinea pig, by specific immunohistochemical methods. In the pancreatic islets, most of insulin-IR cells were detected in the central region, partially in mantle region. Glucagon-IR cells were located in...