The phylogenetic positions of the porcine mycoplasmas Mycoplasma hyosynoviae and Mycoplasma hyopharyngis were determined by using PCR-amplified 16S rRNA gene sequences. M. hyosynoviae is a member of the Mycoplasma hominis group, while M. hyopharyngis belongs to the Mycoplasma fermentans group of mollicutes. Neither species is closely related to previously characterized porcine mycoplasmas belon...

Ensuring mycoplasma-free cell culture is of prime importance as they severely affect cellular characteristics leading to experimental artefacts and spurious results. Various methods persist for mycoplasma detection; out of the whole array of methods polymerase chain reaction (PCR) is the most favoured one because it is highly sensitive, specific and quick. The PCR-based detection procedure invo...

Technologies of artificial insemination cattle are actively used in livestock farms Russia. At the same time, quality control semen production does not involve testing bull for microorganisms genus Mycoplasma. The active use molecular genetic methods allows rapid determination and species identification pathogenic mycoplasmas; however, it indicate viability identified pathogen. paper aims to st...

background and objectives: mycoplasma arthritidis mitogen (mam) superantigen has been shown to induce chronic arthritis, which resembles human rheumatoid arthritis (ra) in a rodent model. however, its role as a causative agent in human ra is not well understood yet. the aim of this study was to investigate the presence of mam superantigen gene in the synovial fluid (sf) of ra patients. material...

Background: Pneumonia due to Mycoplasma infections can cause serious health problems and economic losses in small ruminants industry. Aims: The aim of this study was isolation and identification of Mycoplasmas in sheep naturally infected with pneumonia in Northeastern Iran. Methods: This study used histopathology, culture and ...

i n a survey of pneumonia due to mycoplasma 282 out of 12168 ovine and caprine lung condemnation were collected (2.32%). mycoplasma spp. has been isolated from pneumonic cases in 4 sheep and 2 goats. pcr studies were confirmed the genus of mycoplasma although attempting for identification of strains m. mycoides, m. capricolum/caprine pleuropneumonia and m. arginin were in failure. the lesions i...

ten field isolates of mycoplasma gallisepticum (mg) from different geographical areas of iran were analyzed by random amplification of polymorphic dna (rapd). rapd analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. mg isolates compared with reference strains (s6 and mg ss) and vaccine strain (ts-11) and demonstrated d...

  I n a survey of pneumonia due to Mycoplasma 282 out of 12168 ovine and caprine lung condemnation were collected (2.32%). Mycoplasma spp. has been isolated from pneumonic cases in 4 sheep and 2 goats. PCR studies were confirmed the genus of Mycoplasma although attempting for identification of strains M. mycoides, M. capricolum/caprine pleuropneumonia and M. arginin were in failure. The lesions...

BACKGROUND Mycoplasmas, the smallest and simplest prokaryotes that reside in endosomes of mammalian cells, are widespread contaminants found in cell cultures. About 30% of all cell cultures, varying from 15 to 80%, are reportedly contaminated with mycoplasmas. Here, we present our experience in successfully detecting and treating mycoplasmal infection in various cell lines. METHODS The nested...