background: sparganosis is caused by the invasion of spirometra sparganum into various tissues/organs. subcutaneous sparganosis can be diagnosed by biopsy , while visceral/cerebral sparganosis is not easy to be diagnosed. the diagnosis de-pends largely on the detection of specific anti-sparganum antibodies. t he specific-ity of the elisa could be increased by using s. mansoni sparganum excretor...

Background A specific feature of the autoimmune response in rheumatoid arthritis (RA) is presence anti-citrullinated protein antibodies (ACPAs) patient sera. These can appear several years before disease onset and are involved development RA. Objective We performed proteomic analysis by mass spectroscopy to identify novel citrullinated antigens autoantibodies RA patients. Methods Polypeptides i...

background: dicrocoelium dendriticum, a liver trematode of herbivores, and man is very prevalent among ruminants of iran, resulting in causing significant economic losses. recently its phenotype polymorphism has been found in iran, a phenomenon that may affect the protein profile of the parasite. objectives: the aim of this study was to determine the somatic protein profiles of d.dendriticum in...

Tuberculosis (TB) is a zoonotic infectious disease common to humans and animals which has been caused by a rod shaped, acid fast bacterium, called Mycobacterium bovis. The rapid and sensitive detection is a great challenge for TB diagnosis. The virulent strains of Mycobacterium tuberculosis complex (MTBC) have 16 different regions of difference (RD) in their genome which encode some important a...

Abstract A cognate antigen engagement of TCR induces a conformation change in the CD3 complex (CD3Δc) that is required for productive T cell activation. CD3Δc fails when TCRs interact with poorly immunogenic antigens, like many tumor-associated-antigens (TAAs), which do not active cells. Anti-CD3 monovalent Fab fragments (Mono-Fabs) bound to mimic CD3Δc, producing “co-potentiation” associated w...

Background: The therapy of leishmania infection is difficult and each year 1.5 million new cases of cutaneous leishmaniasis and 500,000 new cases of visceral leishmaniasis are estimated, therefore, there is a need for an effective vaccine. Monoclonal antibody (mAb) is one of the suitable methods for isolation and purification of leishmania antigens. In this report, we produced several mAb aga...

Cationization of BSA generates a molecule that mounts antibody responses of increased magnitude and duration and induces T cell proliferation at concentrations 500 times less than native BSA (nBSA). To explain the alteration in immunogenic properties of this Ag, the uptake of nBSA and cationized BSA (cBSA) by splenic APC has been investigated. T cell proliferation assays were conducted with nBS...

Although ant ibodies against nuclear antigens are considered the hal lmark of systemic lupus e ry thematosus (SLE) 1, antibodies against cytoplasmic consti tuents have also been identified (1, 2). Despite their recogni t ion more than 25 years ago, the prevalence, antigenic specificities, and disease associations of antir ibosomal antibodies (ARA) are all controversial (3-10) . Since these prob...

background: mixed infection with amphistomes seems common in native cattle of iran. the aim of this study was to determine diagnostic antigens in cattle mixed amphistomiasis. methods: specific antigens of cotylophoron cotylophorum , gastrothylax crumenifer and paramphisto­mum cervi (mixed infection), the most common species, were collected from cattle was deter­mined. adult trematodes were coll...