Antiviral proteins (AVP), present in silkworm fecal matter, show activity against nuclear polyhedrosis virus (NPV) in vitro and in vivo. The extract of silkworm fecal matter prepared in phosphate buffer solution of pH 7.5 was subjected to 50% solid ammonium sulfate precipitation to enrich AVP, then which was dialyzed. The dialysate was applied to the column containing silica gel-G, the column e...

For low protein concentrations containing biological samples (in proteomics) and for non proteinaceous compound assays (in bioanalysis), there is a critical need for a simple, fast, and cost-effective protein enrichment or precipitation method. However, 2,2,2-trichloroacetic acid (TCA) is traditionally used for protein precipitation at ineffective concentrations for very low protein containing ...

Calcium carbonate is an important component in exoskeletons of many organisms. The synthesis of calcium carbonate was performed by mixing dimethyl carbonate and an aqueous solution of calcium chloride dihydrate. The precipitation product was characterized by means of scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrar...

Evidence confirming the identity of fibrinogen-precipitating factor and streptococcal M protein is provided by the demonstration of bactericidal, mouse protective, and long chain-producing antibodies in the sera of rabbits immunized with washed M-fibrinogen precipitates. Two precipitin lines were observed in immunoelectrophoresis of human plasma vs. rabbit anti-M-fibrinogen antiserum; no precip...

Tobacco is widely used as a model plant for feasibility studies of recombinant protein production from transgenic plants. However, dealing with large quantities of biomass to recover recombinant proteins is a challenge for down-stream processing. In this study, the effect of isoelectric precipitation on native tobacco protein was first studied. Among the three acids studied, hydrochloric acid i...

This presentation will cover much the same ground as Professor Laurell's but from a slightly different viewpoint. There is no great difference between us, though we use agarose gel electrophoresis only to detect paraproteins. Like others, we feel that scanning the electrophoretogram contributes nothing to the interpretation of the test', as even with high resolution systems the various fraction...

antiviral proteins (avp), present in silkworm fecal matter, show activity against nuclear polyhedrosis virus (npv) in vitro and in vivo. the extract of silkworm fecal matter prepared in phosphate buffer solution of ph 7.5 was subjected to 50% solid ammonium sulfate precipitation to enrich avp, then which was dialyzed. the dialysate was applied to the column containing silica gel-g, the column e...

egg-laying hens were immunized with the echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. the chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (peg) and ammonium sulphate precipitation method, chloroform extraction and the lithium sulphate precipitation method. these methods were com...

For the preparation of proteins for proteome analysis, precipitation is frequently used to concentrate proteins and to remove interfering compounds. Various methods for protein precipitation are applied, which rely on different chemical principles. This study compares the changes in the protein composition of human blood platelet extracts after precipitation with ethanol (EtOH) or trichloroacet...