We discovered that two physiologically occurring metabolic intermediates, glyceraldehyde phosphate and succinate, are potent insulin secretagogues. No other glycolytic intermediate besides glyceraldehyde phosphate was insulinotropic. Succinate, when added to islets as either its monomethyl or dimethyl ester to increase its cellular permeability, was also insulinotropic. In islets, as in other c...

The metabolic processing of dehydrophos, a broad-spectrum peptide antibiotic containing an unusual vinyl-phosphonate moiety, was examined by using a panel of Salmonella enterica mutants deficient in peptide uptake and catabolism. Dehydrophos bioactivity is lost in opp tpp double mutants, demonstrating a requirement for uptake via nonspecific oligopeptide permeases. Dehydrophos bioactivity is al...

Sucrose is an abundant, cheap, and renewable carbohydrate which makes it attractive feedstock for the biotechnological production of chemicals. Escherichia coli W, one few safe E. strains able to metabolize sucrose, was examined pyruvate. The repressor csc regulon deleted in W expressing a variant E1 component pyruvate dehydrogenase complex, these were screened shake flask culture formation fro...

The 2-oxoglutarate dehydrogenase complex constitutes a mitochondrially localized tricarboxylic acid cycle multienzyme system responsible for the conversion of 2-oxoglutarate to succinyl-coenzyme A concomitant with NAD(+) reduction. Although regulatory mechanisms of plant enzyme complexes have been characterized in vitro, little is known concerning their role in plant metabolism in situ. This is...

Multicomponent reactions involving azines (4-methylpyridine, isoquinoline and N-methyl imidazole) and acetylenic ester were undertaken in the presence of CH compound such as 2-furoyl trifluoroacetone(4,4,4-trifluoro-1-thiophen-2-yl-butan-1,3-dione) to generate enaminoesters as stable 1,4-diionic compounds in high yields.

Using isolated hepatocytes from fasted rats, the oral hypoglycemic agents, Z-tetradecylglycidic acid (McN3802) and its methyl ester (McN-3716) inhibited (concentrations down to 5 x lo-’ M) the oxidation of palmitate to CO2 and ketones but not the oxidation of octauoate or palmitoylcarnitine. The antiketogenic effect which occurs at concentrations as low as lo-’ M was accompanied by a lowered P-...

background: the frequency of pyruvate kinase (pk) deficiency, an autosomal recessive defect, is approximately 3 per 10,000 individuals in shiraz and surrounding areas, and is increased due to high consanguinity marriage frequency. the purpose of this study is to obtain data on the frequency and spectrum of gene mutation of pk in newborns, from shiraz and surrounding areas. materials and methods...

Hydrogen isotope ratios of plant lipids are used for paleoclimate reconstruction, but influenced by both source water and biosynthetic processes. Measuring 2H : 1H multiple compounds produced different pathways could allow these effects to be separated, hydrogen fractionations during isoprenoid biosynthesis remain poorly constrained. To investigate how fractionation is molecular exchange betwee...

Hyperpolarization methods in magnetic resonance enhance the signals by several orders of magnitude, opening new windows for real-time investigations dynamic processes vitro and vivo. Here, we propose a field-independent para-hydrogen-based pulsed method to produce rapidly hyperpolarized 13C-labeled substrates. We demonstrate polarizing carboxylic carbon pyruvate moiety purposely designed precur...