Reverse transcription of RNA is an invaluable method for gene expression analysis by real-time PCR or microarray methods. Random primers of varying lengths were compared with respect to their efficiency of priming reverse transcription reactions. The results showed that 15-nucleotide-long random oligonucleotides (pentadecamers) consistently yielded at least 2-fold as much cDNA as did random hex...

in recent years, some statisticians have studied the signal detection problem by using the random field theory. in this paper we have considered point estimation of the gaussian scale space random field parameters in the bayesian approach. since the posterior distribution for the parameters of interest dose not have a closed form, we introduce the markov chain monte carlo (mcmc) algorithm to ap...

The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served...

to investigate the genetic diversity of 21 rapeseeds (brassica napus l.), 20 semi-random isj and ssr primers were used. primers produced a total number of 158 (81.5%) polymorphic bands (51 ssr and 107 isj bands). average numbers of polymorphic bands for isj, ssr and combined of them were 10.7, 5.1 and 7.9, respectively. pic values ranged from 0.0150 for specific primer o110d08 to 0.3420 for sem...

Background and Objective: Escherichia coli bacteria are one of the most important bacterial races causing urinary tract infection, which is responsible for 90% causes infection originated in human intestine, this Gram- Negative facultative aerobic that moves by peripheral flagella belongs to Enterobacteriaceae.
 Materials methods: The were isolated from UTI, after they diagnosed using ligh...

Forensic entomology is the study of insects found around the crime scene and are helpful for the estimation of time elapsed since death of a person i.e., post mortem interval (PMI). There are number techniques available for the identification of insect species and Random Amplified Polymorphic DNA (RAPD) is one of those. RAPD is a useful technique designed for the detection of sequence polymorph...

consider the random walk among n places with n(n - 1)/2 transports. we attach an exponential random variable xij to each transport between places pi and pj and take these random variables mutually independent. if transports are possible or impossible independently with probability p and 1-p, respectively, then we give a lower bound for the distribution function of the smallest path at point log...