Brazil harbours a unique ecosystem, the Caatinga, which belongs to the tropical dry forest biome. This region has an important diversity of organisms, and recently several new fungal species have been described from different hosts and substrates within it. During a survey of fungal endophyte diversity from cacti in this forest, we isolated cladosporium-like fungi that were subjected to morphol...

Morphological studies and phylogenetic analyses of DNA sequences from three genomic regions - the internal transcribed spacer (ITS) regions of the nuclear ribosomal gene repeat, a partial sequence of RNA polymerase II subunit (rpb2), and a partial sequence of translation elongation factor (tef1) - were used to investigate the systematics of Hypocrea citrina and related species. A neotype specim...

We sampled and analyzed approximately 2900bp across the three loci from 54 taxa belonging to a taxonomically difficult group of Cortinarius subgenus Phlegmacium. The combined analyses of ITS and variable regions of RPB1 and RPB2 greatly increase the resolution and nodal support for phylogenies of these closely related species belonging to clades that until now have proven very difficult to reso...

T structure of 10-subunit 0.5-MDa yeast RNA polymerase II (pol II), recently determined at 2.8 Å resolution, reveals the architecture and key functional elements of the enzyme (1). The two largest subunits, Rpb1 and Rpb2, lie at the center, on either side of a nucleic acid-binding cleft, with the many smaller subunits arrayed around the outside. Rpb1 and Rpb2 interact extensively in the region ...

Approximately 3000 bp across 84 taxa have been analyzed for variable regions of RPB1, RPB2, and nLSU-rDNA to infer phylogenetic relationships in the large ectomycorrhizal mushroom genus Inocybe (Agaricales; Basidiomycota). This study represents the first effort to combine variable regions of RPB1 and RPB2 with nLSU-rDNA for low-level phylogenetic studies in mushroom-forming fungi. Combination o...

X-ray diffraction data from two forms of yeast RNA polymerase II crystals indicate that the two largest subunits of the polymerase, Rpb1 and Rpb2, may have similar folds, as is suggested by secondary structure predictions. DNA may bind between the two subunits with its 2-fold axis aligned to a pseudo 2-fold axis of the protein.

Methods Twenty-eight populations (210 individuals) were sequenced for one nuclear (rpb2) and two chloroplast (trnL–F and psbA–trnH) DNA markers. Intraspecific phylogenetic relationships among haplotypes were reconstructed, and molecular dating, population genetic analyses and group testing were performed on the data. Isolation-by-distance analysis was conducted for the populations spanning the ...

Abstract A major fungal lineage of myxacioid taxa is centred around Cortinarius vibratilis . This shown by morphological and multigene molecular phylogenetic analyses the nrDNA ITS LSU, as well rpb1 rpb2 genes to form a well‐supported clade, here introduced new subgenus C. subg. Vibratiles consisting four sections various small clades. Two sections, sect. Microspermi Turcopedes , are also propo...