Soybean somatic embryos have attracted attention both as a model of zygotic embryos and as explants for the generation of stable transgenic plants. We have now characterized the maturation of soybean somatic embryos in detail by examining both the accumulation of the major seed storage proteins b-conglycinin and glycinin as well as changes in cellular organization. Protein storage vacuoles and ...

Background: For several years, mammalian cloning by splitting an early embryo or nuclear transfer into oocytes method has been successfully performed. Cloning is now also possible using adult somatic cells. Although it has now been 15 years since the first cloned mammals were generated from somatic cells using nuclear transfer (NT), the success rate for producing live offspring by cloning is lo...

Somatic embryogenesis has been shown to be an efficient in vitro plant regeneration system for many crops such as the important ornamental plant Cyclamen persicum, for which this regeneration pathway of somatic embryogenesis is of interest for the vegetative propagation of parental lines as well as elite plants. However, somatic embryogenesis is not commercially used in many crops due to severa...

Dynamic changes in callose content, which is deposited as a plant defense response to physiological changes, were analyzed during somatic embryogenesis in Eleutherococcus senticosus zygotic embryos plasmolyzed in 1.0 M mannitol. During plasmolysis, callose deposition was clearly observed inside the plasma membrane of zygotic embryo epidermal cells using confocal laser scanning microscopy. The c...

A method has been developed for plant regeneration by encapsulation of somatic embryos obtained from callus cultures derived from semi-mature cotyledon explants of Dalbergia sissoo Roxb. (family Fabaceae). Embryogenic callus was developed from cotyledon pieces on Murashige and Skoog (1962) medium supplemented with 9.04 μM 2,4dichlorophenoxyacetic acid and 0.46 μM kinetin. The somatic embryos we...

this study was carried out to investigate the morphological and anatomical aspects of somatic embryogenesis in date palm. lateral bud and shoot tip explants excised from young offshoots were cultured on ms medium with 2,4-d. somatic embryogenesis was in-duced by transferring the calli produced on the same medium without hormones. micro-tome sectioning of paraffin-embedded specimens was carried ...

Having produced the embryos of cactus Copiapoa tenuissima Ritt. forma monstruosa at the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100  μ M on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process we also investigated a combined effect of ABA ...

Many factors may influence the efficiency of somatic embryogenesis. This capability may be differ with regard to media compositions, genotype, tissue, organ ontology and the stage of differentiation. The effects of five salicylic acid concentrations (0, 25, 50, 75 and 100 mM) on different stages of carrot somatic embryogenesis were studied using petiole and root secondary phloem explants as sta...

Neem is a highly heterozygous and commercially important perennial plant. Conventionally, it is propagated by seeds which loose viability within two weeks. Strictly cross pollinating nature of the plant causes serious barrier to the genetic improvement by conventional methods. Alternative methods of tree improvement such as somatic hybridization, mutagenesis and genetic transformation require a...