Background and Purpose: Aflatoxins are naturally produced by some species of Aspergillus, such as A. flavus and A. parasiticus. Aflatoxins reportedly have carcinogenic effects on human, poultry, and livestock, and therefore could be linked to severe human illnesses. Aflatoxin biosynthesis pathway involves different clustered genes, including structural, regular, and unassigned genes. The presen...

Leishmaniasis include several clinical manifestations, mostly cutaneous, visceral and mucosal (1, 2). Various species can lead to diverse clinical presentations. Thus species identification contribute to proper management (3). Localized Leishmania Lymphadenitis (LLL) is a distinct entity in clinicopathologic field presenting with isolated lymphadenitis and possible cocomitant cutaneous...

In order to improve simultaneous detection and identification of Helicobacter genus in general and Helicobacter pylori specifically and reduce the number of amplifications needed, we established a multiplex PCR. In this study, two pairs of primers: Hcom1 and Hcom2 specific for Helicobacter genus, Hicd1 and Hicd2 specific for Helicobacter pylori species were used. To determine the sensitivity of...

background: a. bovis and a. phagocytophilium are leukocytotropic agents of bovine anaplasmosis. they are obligate intracellular bacteria that can infect and cause anaplasmosis in human and animals. therefore, this study was carried out to detect a. bovis and a. phagocytophilum in naturally infected dairy cattle in isfahan using molecular techniques. materials and methods: in this study a total ...

the genus entamoeba contains many species, six of which (entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, entamoeba polecki, entamoeba coli, and entamoeba hartmanni) reside in the human intestinal lumen. entamoeba histolytica is the causative agent of amebiasis in humans with a worldwide distribution. correct detection of e.histolytica is frequently a major concern in diagnostic ...

Figure 1: Rare Event Detection includes both Rare Mutation Detection (RMD) and Rare Species Detection (RSD). RMD and RSD can be classified according to their assay components. An RMD assay is comprised of a single primer set plus two competitive probes. RSD utilizes either a single assay to detect a rare species per unit volume (Case 1) or two independent, noncompetitive assays to detect a rare...

leishmaniasis include several clinical manifestations, mostly cutaneous, visceral and mucosal (1, 2). various species can lead to diverse clinical presentations. thus species identification contribute to proper management (3). localized leishmania lymphadenitis (lll) is a distinct entity in clinicopathologic field presenting with isolated lymphadenitis and possible cocomitant cutaneous lesion i...