methods allplex gi-virus assay was developed to detect pathogens causing viral gastroenteritis, one of the major diseases caused by rna viruses. this one-step multiplex real-time polymerase chain reaction (pcr) based on the mudt technique permits simultaneous amplification and detection of target nucleic acids of norovirus gi, norovirus gii, rotavirus a, adenovirus f, astrovirus, and sapovirus ...

in recent research on modelling road collisions very little attention has been paid  to rear-end chain reaction collisions, which is characterized by more than two vehicles involved in a collision at the same time. the core aim of the present research is to develop a methodology to estimate such potential collision probabilities based on a proactive perspective, where deceleration rate to avoid...

background and objectives: hbv dna monitoring is important in management of chronic viral hepatitis b infection. hbv dna measurements are carried out over period of months to years. so the analytical system must be stable and reproducible. the aim of this study was to determine the performance characteristics and to plan a statistical quality control system of a laboratory-developed real-time q...

conclusions our results demonstrated a novel h7n9 virus in chickens in taizhou city in 2013 that may pose a potential risk to public health. results of 150 samples tested, 7 were detected to be h7n9 positive which all were collected from chickens. also, a novel h7n9 virus strain (a/chicken/jiangsu/1021/2013) was isolated and studied. phylogenetic analysis showed the novel isolated h7n9 virus ha...

vaccines is considered as a critical point and in-process of quality control (ipqc) test of vaccine production. rapid tests, like real time pcr, are more appropriated when the production occurs at industrial scale because of the amounts of starting materials and the excess of consumed time required. in the current study, a real-time quantitative reverse transcription-polymerase chain reaction (...

conclusions this study showed that this novel one-step rrt-pcr assay is a rapid, reliable, repeatable, specific, sensitive, and simple tool for the detection of the cchf virus. results from a total of 75 suspected serum samples, 42 were confirmed to be positive for cchf virus, with no false-positives detected by the sequencing results. after 40 amplification cycles, the melting curve analysis r...