Aim. To reveal the associations of IgA antibodies to benzo[a]pyrene, estradiol and progesterone (IgA-Bp, IgA-Es, IgA-Pg) with conversion estrogen-receptor positive (ER+) into negative (ER-) tumors during breast cancer progression. Materials Methods. Having collected serum samples from 338 healthy volunteers 1407 patients, we have profiled them for IgA-Bp, IgA-Pg by means enzyme-linked immunosor...

The water-soluble carbodiimide salt 1-ethyl-3-(3'-dimethylaminopropyl)carbodiimide. HCl (EDCI-HCl) has been shown to increase the complement-dependent lysis of cultured mouse neuroblastoma C1300 cells by two types of antibody: (1) natural antibodies in the sera of normal (nonimmunized) rabbits, and (b) serum antibodies from snygeneic tumor-bearing A/HeJ mice. In the latter case, both the level ...

SUMMARY OBJECTIVE: Currently, there is an ongoing debate whether progesterone receptor positive and estrogen negative breast carcinomas represent a true distinct subtype of tumor or mere immunohistochemical artifact. In this study, we conducted immunohistochemistry panel with the antibodies TFF1, EGFR, CK5 to reclassify phenotype in luminal basal-like subtype. METHODS: Tumors -/progesterone +, ...

BALB/c mice immunized with syngeneic methylcholanthrene-induced fibrosarcomas did not have antibodies against the unique tumor-specific transplantation antigens, even though they were capable of rejecting a lethal dose of tumor cells. Endogenous murine leukemia virus antigens expressed by certain of the tumors did, however, elicit high titers of antibodies, accounting for serological crossreact...

Monoclonal anti-idiotype antibodies can be made which are exquisitely specific for B lymphocytic malignancies. We have conducted a clinical trial in which some patients' tumors regressed after infusion of such antibodies. Here, we evaluated characteristics of the antibodies, the tumors, and the patients to determine which features best correlated with the clinical response. Neither the isotype ...

Abstract Many studies have confirmed that the human poliovirus receptor (PVR; CD155) is related to tumor cell migration, invasion, and thus progression. A PVR binds its ligand T Ig ITIM domain (TIGIT) inhibit function of NK cells, thereby allowing tumors evade immune surveillance. In this study, two IgG1 monoclonal antibodies, anti-CD155 anti-TIGIT, were expressed by mammalian transient transfe...

OX40 is a costimulatory molecule that belongs to the tumor necrosis factor receptor (TNFR) superfamily. agonist-based combinations are emerging as promising candidates for novel cancer immunotherapy. Clinical trials have shown agonist antibodies could lead better results in patients. Using hybridoma platform and three different types of immunization strategies, namely recombinant protein, DNA, ...

BACKGROUND Human papillomavirus (HPV) is a risk factor for head and neck cancers (HNC), yet HPV-associated tumors have better prognosis than HPV-negative tumors. METHODS We evaluated whether pretreatment presence of antibodies to HPV capsids [virus-like particles (VLP)] or to HPV-16 oncoproteins E6 and E7 was a predictor of HPV-positive HNC and clinical outcomes. Sera from 156 HNC patients we...

BACKGROUND Patients with neuroendocrine-related tumors and paraneoplastic encephalomyelitis (PEM) or paraneoplastic sensory neuronopathy (PSN) develop high titers of antibodies, called anti-Hu, against neuronal proteins expressed in their tumors, usually small cell lung cancer (SCLC). These tumors appear to be more indolent than those not associated with anti-Hu antibodies. The aims of this stu...

PURPOSE Epidermal growth factor receptors (EGFR) play an important role in tumorigenesis and, therefore, have become targets for new molecular therapies. Here, we use a "cocktail" of optically labeled monoclonal antibodies directed against EGFR-1 (HER1) and EGFR-2 (HER2) to distinguish tumors by their cell surface expression profiles. EXPERIMENTAL DESIGN In vivo imaging experiments were done ...