RNA isolation is performed everyday by molecular biologists for a wide range of applications. No matter what protocol is used and how meticulous the user is, a risk to degrade RNA during the process or to carry over contaminants always exists which may affect the reliability of the subsequent analytical techniques. The parameters that are usually checked for quality control include the concentr...

Background: The prevailing methods for the genomic DNA extraction of Sclerotium rolfsii from its mycelium mat is often time consuming and yields poor quality quantity owing to presence outrageous magnitude mucilage polysaccharides. Methods: A new fast track method isolation resting structure (sclerotia) S. by modifying CTAB deprived supplementation proteinase K has been standardized. Result: pr...

Arils from six pomegranate (Punica granatum L.) varieties grown in the Mediterranean region of Turkey were tested for their antimicrobial properties by the agar diffusion and minimum inhibitory concentration (MIC) methods against seven bacteria: (Bacillus megaterium DSM 32, Pseudomonas aeruginosa DSM 9027, Staphylococcus aureus Cowan 1, Corynebacterium xerosis UC 9165, Escherichia coli DM, Ente...

In this study flowering time and other important morphologic traits were evaluated during two years in F1 almond progenies of seventy two seedlings from cross between the intermediate flowering Italian cultivar ‘Tuono’ and the extra-late blooming Iranian cultivar ‘Shahrood-12’. A modified-bulk segregant analysis in combination with the application of the 140 RAPD primers, 87 nuclear ...

Almond oil is used in many cosmetic products as a snack, in confectionery (marzipan, "turrón", nougat), food products (almond milk, ice cream, chocolate), culinary recipes and also cosmetic base. For surveying the effects of almond genotype and growing location on oil percentage of oil seed content and fatty acid of almond, seeds of 6 almond cultivars: Ferragnes, Tuono, Azar, Sahand, Nonpareil ...

Drought stress is the most important factor limiting successful production of agricultural crops worldwide. The response some almond cultivars grafted on GF677 rootstock was studied to drought at a research greenhouse in Horticultural Science Research Institute Karaj, Iran, 2016-2017 growing season. plant materials included 12 (Prunus dulcis Mill.) genotypes/cultivars, including ‘Sh10’, ‘Saba’,...

Soil analysis is crucial for estimating the risk of crop damage by root-knot nematode Meloidogyne hapla. Here, we developed an assay based on Loop-mediated Isothermal Amplification (LAMP). The LAMP primers were verified specificity against 10 different species. A manual soil DNA extraction, referred to as SKMM, was and compared with a FastDNA kit followed purification. extracted both methods fr...

self-incompatibility is one of the main problems in almond production. in this research work, at the first step, some local cultivars as well as some imported ones were evaluated from the point of their male and female organ compatibility. then, ten selected commercial self-incompatible cultivars(female parents) were crossed with two self-compatible cultivars(male parents) i. e. tuono and super...