background: lipopolysaccharides (lpss) and several antigenic proteins of brucella have been considered for preparation of diagnostic reagents and subunit vaccines. the objective of this study was to identify and compare immunogens of b. abortus s19 which induce humoral immune responses in human, goat and rabbit.   material and methods: the bacterial whole cell extract was prepared in extraction...

background: glucantime ® is the first- line drug for the treatment of all forms of leishmaniasis. unfortunately, the prevalence of parasites becoming resistant to glucantime ® is increasing in several parts of the world including iran. as protein is the most important target for drugs in response to a variety of signals including drugs so, it seems expression protein patterns in sensitive and r...

Two-dimensional gel electrophoresis (2DGE) is the leading technique to separate individual proteins in biological samples with many biological and pharmaceutical applications, e.g., drug development. The technique results in an image in which the proteins appear as dark spots on a bright background. The analysis of these images is very time consuming and requires a large amount of manual work. ...

Two-dimensional electrophoresis (2-DE) is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. This technique sort’s protein according to two independent properties in two discrete steps: the first-dimension step, isoelectric focusing (IEF), separates proteins according to their isoelectric points (pI); the se...

Plasma/serum protein patterns in human fetuses and infants: a study by high-resolution two-dimensional polyacrylamide gel electrophoresis. Applied and Theoretical Electrophoresis 3: 183}190. Tissot JD and Spertini F (1995) Analysis of immunoglobulins by two-dimensional gel electrophoresis. Journal of Chromatography A 698: 225}250. Tissot JD, Invernizzi F, Schifferli J, Spertini F and Schneider ...

conclusions our successful extraction of purified lps from e. coli isolates of uti patients’ urine samples can be an important step to understand the uti disease conditions. results the silver-stained gel demonstrated both smooth and rough type lps by showing trail-like band patterns with the presence and lacking o-antigen region, respectively. coomassie blue staining showed no band assuring th...

The mitochondrial polypeptides from maize lines susceptible and resistant to Helminthosporium maydis were studied by single- and two-dimensional gel electrophoresis. Approximately 120 polypeptides were detected by two-dimensional gel electrophoresis. However, it was not possible to detect qualitative differences between the mitochondrial polypeptides of the two inbreds. These observations are d...

Consistent differences in expression of specific proteins were observed between numerous cancer cell clones with high and low potential for spontaneous metastasis. Seventeen clones from two unrelated murine fibrosarcomas were examined concomitantly for spontaneous formation of lung metastases and for occurrence of individual polypeptide differences by high resolution two-dimensional gel electro...