The aim of this study was to analyse semen quality parameters fresh-extended porcine imported from Denmark through prolonged importing regulations and transport fertility performance in sows inseminated four days post collection. Semen analysed immediately upon the arrival (4 collection) using computer-assisted sperm analyses (CASA), cytomorphology flow cytometry, were later same day. mean conc...

AIM To study the effect of preputial washing on bacterial load, preservability and semen quality in Murrah buffalo bulls. MATERIALS AND METHODS A total of 36 collections of three Murrah buffalo bulls maintained at Artificial Breeding Research Centre, ICAR-National Dairy Research Institute, Karnal, were collected at weekly intervals from each bull without preputial washing and latter ejaculate...

background: cryopreservation of semen requires optimized conditions to minimize the harmful effects of various stresses. the main approach for protection of sperm against stress is based on the use of antioxidants and cryoprotectants, which are described as defensive methods. recently, the application of controlled mild stressors has been described for activation of a temporary response in oocy...

Cryopreservation of spermatozoa is a pivotal tool in assisted reproduction, and studies aiming to establish optimal freezing/thawing protocols are essential to enhance sperm survival. The objectives of the present study were to (1) compare the cryoprotective efficiency of three different glycerol concentrations (3%, 5%, and 7%) on the basis of post-thaw sperm quality and (2) investigate whether...

A good functional status of cryopreserved boar spermatozoa is very important for successful fertilization of porcine oocytes and in vitro embryo production. The purpose of the study was to evaluate the changes in functional status of boar spermatozoa separated from frozen-thawed semen and capacitated in vitro by caffeine. The effect of acrosome reaction development in spermatozoa on the efficie...

To achieving high efficiency in sheep, ram sperm must have fertilization ability. Quercetin, a potent antioxidant, has been shown to positive effect on semen different species. This study aims improve the of Merino using quercetin. The ejaculates collected from rams were used for dilution (sperm density ≥ 3×109 spermatozoa/ml, motility 75%). Tris diluent was dilute semen. divided into 4 equal v...

We consider images of boar spermatozoa obtained with an optical phase-contrast microscope. Our goal is to automatically classify single sperm cells as acrosome-intact (class 1) or acrosome-reacted (class 2). Such classification is important for the estimation of the fertilization potential of a sperm sample for artificial insemination. We segment the sperm heads and compute a feature vector for...

Spermatozoa sexing requires a diluent that can protect and provide an optimal environment to maintain the quality of spermatozoa. This study targets determine effectiveness adding sucrose tris-egg yolk on sperm swamp buffaloes produced by with egg albumin method. Semen was collected once week for 12 consecutive weeks artificial vagina from three male at Indonesian Center Animal Research Develop...

The purpose of this study was to examine if selecting a sperm population with improved motion characteristics before freezing reduces the deleterious effects of cryopreservation. Semen specimens from 15 normal donors were divided into two equal aliquots. The first aliquot received no treatment (control), and the second was processed by swim-up from a washed sperm preparation to select a sperm p...