OBJECTIVE To evaluate cryodamage on human sperm motility, cryosurvival rate, and sperm chromatin assessed by acridine orange staining method (AO test) after a six-month freeze-thawing process using liquid nitrogen vapor. STUDY DESIGN Experimental study. MATERIAL AND METHOD Twenty normal semen samples were obtained from the male partner of infertile couples attending the infertility unit, Si...

The ability to cryopreserve lymphocytes in peripheral blood mononuclear cells (PBMC) to retain their function after thawing is critical to the analysis of cancer immunotherapy studies. We evaluated a variety of cryopreservation strategies with the aim of developing an optimized protocol for freezing and thawing PBMC to retain viability and function. We determined several factors which do not af...

The purpose of this study was to determine the negative effects (cryodamage) on human spermatozoa after freeze-thawing and to determine whether freeze-thawing of spermatozoa with a programmed slow freezer is better than freezing with liquid nitrogen vapour (rapid freezing) with regard to alterations in sperm chromatin and morphology in semen from fertile (donor) and subfertile, IVF/ICSI, patien...

The objective was to determine the effect of sequence of insemination after simultaneous thawing of multiple 0.5 mL semen straws on conception rate in suckled multiparous Nelore cows. The effect of this thawing procedure on in vitro sperm characteristics was also evaluated. All cows (N = 944) received the same timed AI protocol. Ten straws (0.5 mL) of frozen semen from the same batch were simul...

objective: vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. however, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. this study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mo...

Increasing use of frozen storage in nurseries at northern latitudes calls for thawing methods that are safe, economical and easy to apply on a large scale. The easiest and most economical method would be to thaw seedlings in the same boxes they were stored in. However, doing this safely requires more knowledge about how long and at what temperatures seedlings should or can be kept in the boxes ...

The study was conducted to determine the influence of D-aspartic acid (D-Asp) as antioxidant supplement and thawing rates on ram sperm motility, membrane integrity, abnormality, viability, mitochondria activity, malondialdehyde and antioxidant activities, and total antioxidant capacity after freezing-thawing process. Semen samples from five mature rams (3-4 years old) were diluted with extender...

The aim of this study was to determine whether more than once freezing and thawing of serum affects different patterns of anti-nuclear antibodies (ANA) and anti-neutrophil cytoplasmic antibodies (ANCA) as also to determine for how many days can serum samples be reliably refrigerated (2-8oC) for these tests. A total of 20 ANA (6 homogenous, 3 speckled, 3 centromere, 2 nucleolar, 2 cytoplasmic an...

introduction: this study was designed to compare the effects of three vitrification procedures [conventional (c), open pulled straw (ops) and closed pulled straw (cps)] on morphological survival of two-cell mouse embryos and their subsequent development to hatched blastocysts. material and methods: two-cell mouse embryos were divided into four groups, control (194), ops (150), cps (155) and c (...