Previous electrophoretic methods for the separation of tissue-specific serum alkaline phosphatases have either been unable to separate the liver and bone enzymes or have been too involved for routine clinical use. A relatively simple electrophoretic method is described which separates placental, liver, bone, and intestinal alkaline phosphatases in serum. The clinical applications of such a meth...

1. The inorganic-pyrophosphatase activity of alkaline phosphatases prepared from human liver and small intestine was investigated at different stages of purification. 2. Both liver and intestinal preparations possessed pyrophosphatase activity at all stages of purification, and the two types of activity were not separated by gel filtration or by anion-exchange or cation-exchange chromatography....

The gene encoding Thermus caldophilus GK24 (Tca) alkaline phosphatase was cloned into Escherichia coli. The primary structure of Tca alkaline phosphatase was deduced from its nucleotide sequence. The Tca alkaline phosphatase precursor, including the signal peptide sequence, was comprised of 501 amino acid residues. Its molecular mass was determined to be 54¿ omitted¿760 Da. On the alignment of ...

We studied several factors affecting biological variation in serum acid phosphatases in a population of 1195 apparently healthy subjects four years old or older. We assayed total acid phosphatase activities in the presence of a transphosphorylating agent and using alpha-naphthyl phosphate as substrate. The main factors modifying total and tartrate-resistant acid phosphatases activities in serum...

Tissue phosphatases which are present in virus suspensions were found to contribute in considerable measure to the inactivation of herpes simplex virus. Phosphatase inhibitors and phosphatase substrates have been found to prolong the survival of the virus. Exogenous phosphatases, both acid and alkaline, have been demonstrated to bring about the loss of infectivity of suspensions of this virus.

A detailed biochemical study has been made in the heart of Pteropus giganteus for glycogen and acid and alkaline phosphatase contents. Highest concentration of glycogen and both the phosphatases was observed in the right atrium and basal region of the ventricles, suggesting more activity in these regions. Apical region of the ventricle represented minimum content of glycogen and phosphatases.

a-Amino acids have been shown to exert a substantial inhibitory effect on the hydrolysis of sodium /3-glycerophosphate by tissue alkaline phosphatases at optimal pH (1). The inhibition by r,-histidine was particularly marked; inhibition constants (Ki) of approximately 6 X 10-S M and 3 X lOma M were obtained for rat intestinal and rat bone phosphatases, respectively (1). The primary concern of t...

Bacterial alkaline phosphatases (APases) are important enzymes in organophosphate utilization in the ocean. The subcellular localization of APases has significant ecological implications for marine biota but is largely unknown. The extensive metagenomic sequence databases from the Global Ocean Sampling Expedition provide an opportunity to address this question. A bioinformatics pipeline was dev...

Alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] in placenta, intestine, liver, kidney, bone, and lung from a variety of primate species has been characterized by quantitative inhibition, thermostability, and immunological studies. Characteristic human placental-type alkaline phosphatase occurs in placentas of great apes (chimpanzee and orangutan)...