urine specimens collected from in-patients and out-patients in urmia imam hospital, northwest iran, were cultured on blood agar and eosin methylene blue agar. isolated bacteria were identified according to standard microbiological tests and then subjected to sensitivity testing according to routine method of disk agar diffusion technique. out of 8044 and 10425 urine specimens, 8.7% and 11.9% we...

Appropriate use of antimicrobials in health care continues to be a challenge. Reliable and reproducible antimicrobial susceptibility testing methods are necessary to provide the clinician with valuable information that can be translated into positive clinical outcomes at the bedside. However, there are nuances with these testing methods that, if unrecognized, could lead to misinterpretation of ...

Infections caused by Chlamydia spp are an important cause of human disease, and the accuracy and reproducibility of antimicrobial susceptibility tests for these bacteria could have considerable clinical implications. We have developed a reverse transcriptase PCR (RT-PCR) based method to determine the antibiotic susceptibility of Chlamydia spp., and compared this with conventional tests using im...

The greatest threat to antimicrobial treatment of infections caused by Gram-negative bacteria is the production of carbapenemases. Metallo-beta-lactamases and plasmid-mediated serine carbepenemases like Klebsiella pneumonia carbapenemase are threatening the utility of almost all currently available beta-lactams including carbapenems. Detection of organisms producing carbapenemases can be diffic...

Abstract Background New technologies offer the promise of faster, more accessible antibiotic susceptibility testing. UK policy includes development and introduction innovative diagnostics to tackle antimicrobial resistance, whilst many new are emerging, we still have limited evidence their performance. Many these emerging changing two core aspects tests (AST). Firstly, using microfluidic platfo...

disk diffusion test is the usual applicable method for assessing the antimicrobial susceptibility pattern in most institutions and hospitals. the aim of this study was to determine the reliability of resistant-reported results of disk diffusion test for 6 routinely used antibiotics against gram-positive microorganisms of nosocomial origin, using e-test method.    over a 1-year period, clinical ...

introduction: preventing and starting early treatment of infections in patients whose immunity system is weak due to malignancies like leukemia can reduce mortality. this study aimed to determine microbial and antibiotic resistance patterns in clinical samples of patients with acute leukemia to start early treatment before the results of clinical tests are known. subjects and methods: in this c...

The increasing prevalence of ciprofloxacin-resistant Neisseria gonorrhoeae has required replacing inexpensive oral ciprofloxacin treatment with more expensive injectable ceftriaxone. Further, monitoring antimicrobial resistance requires culture testing, but nonculture gonorrhea tests are rapidly replacing culture. Since the strategies were similar in effectiveness (> 99%), we evaluated, from th...

INTRODUCTION Antimicrobial resistance is neglected in developing countries; associated with limited surveillance and unregulated use of antimicrobials. Consequently, delayed patient recoveries, deaths and further antimicrobial resistance occur. Recent gastroenteritis outbreak at a children's home associated with multidrug resistant non-typhoidal Salmonella spp, raised concerns about the magnitu...