BACKGROUND Chlorophytum borivilianum is an industrially valued medicinal crop. Propagation through seeds is not feasible because of low germination percentage and long dormancy period. Therefore, callus culture and plant regeneration can be an alternative to improve this crop production. Also, callus can serve as an alternative source of bioactive compounds. OBJECTIVE To evaluate the effect o...

One of the main obstacles in providing quality potato seed tubers is long dormancy period seeds. A study that explores this has been carried out to elucidate response release and initiation sprouts differences tuber weight storage duration before immersion cytokinin (6-benzylaminopurine). The was conducted at warehouse UPT Horticulture Parent Seed Kutagadung Berastagi, Department Food North Sum...

A cytokinin-binding protein has been isolated from wheat germ via ammonium sulfate precipitation, carboxymethyl Sephadex chromatography, and affinity chromatography on a column substituted with a derivative of kinetin riboside. On Sephadex G-200, the protein migrated with an apparent molecular weight of 122,000 daltons. The dissociation constant for kinetin was determined by equilibrium dialysi...

Arrowroot (Maranta arundinacea L.; Marantaceae) is an excellent source of starch (>85%) used in the food industry. The multiplication of this plant is traditionally from rhizomes, which are also a source of starch. In vitro M. arundinacea shoot cultures were successfully established from rhizome buds on semi-solid MS medium supplemented with 3 mg.l 6-benzylaminopurine in the dark, which was als...

The promoters of the three Arabidopsis nuclear genes encoding mitochondrial cytochrome c oxidase subunit 6b (AtCOX6b) have similar expression patterns, with preferential expression in anthers and meristems, and are induced by sucrose and etiolation. Additionally, induction of AtCOX6b-1 by GA(3) and AtCOX6b-3 by 6-benzylaminopurine was observed. Site II elements (TGGGCC/T) present in the three p...

The senescence of excised discs of primary leaves of Phaseolus vulgaris, L., var. Red Kidney was followed by measuring the net breakdown of protein and chlorophyll. The chemical growth regulators indoleacetic acid, 2,4-dichlorophenoxy-acetic acid, gibberellic acid, kinetin, and 6-benzylaminopurine were relatively ineffective in retarding senescence in this tissue. White light, on the other hand...

In order to test the metabolic stability of 9-substituted cytokinins, 6-benzylamino-9-methyl purine has been synthesized and labeled with (14)C in the 9-methyl carbon or doubly labeled with (14)C in the 9-methyl carbon and (3)H in the methylene moiety of the side chain. Although the 6-benzylamino-9-methylpurine is chemically stable, cytokinin-requiring tissues begin removing the 9-substituent i...

The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27oC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regen...

Elms are threatened by Dutch elm disease, and conservation methods needed to protect their genetic diversity. Cryopreservation of dormant buds allows large numbers genotypes be conserved with small space requirements minimal upkeep. through slow controlled cooling was tested for both species native Finland, Ulmus glabra laevis. Regeneration the thawed micropropagation studied on different basal...