A salt-extractable low-molecular-weight fraction has been held responsible for the resistance of cartilage to invasion by malignant cells. To test this hypothesis, we have confronted in vitro fragments from bovine articular cartilage, from bovine nasal septum, from chick embryonic tibia, or from human knee meniscus with cells from the following malignant lines: LICR-HN-4 human squamous cell car...

Recently, a new isoform of the type II transforming growth factor beta receptor (TGF-beta RII) was identified. This isoform (TGF-beta RII2) contains an insertion of 25 amino acids in the extracellular domain of the receptor. Using RT-PCR the authors demonstrated that both TGF-beta RII1 and TGF-beta RII2 are expressed by chondrocytes in murine and human articular cartilage. Bovine articular chon...

Type II collagen is the major collagen of cartilage. However, new collagenous chains have been described in different cartilaginous tissues, recently. The la, 2a and 3a chains were extracted from human and bovine hyaline cartilage [1]. Other minor collagenous components were extracted from neonatal pig and human cartilage noted M, CFI, CF2 [2-4], from bovine nasal cartilage and human interverte...

This study is designed to determine whether the outermost layer of articular cartilage deficient in Osteoarthritis (OA). Phospholipids present healthy and osteoarthritis (OA) synovial fluid show significant differences their concentration. While examining surface properties OA joints, we found that PLs molecules cannot support lubrication, increased friction was observed. Our lubrication mechan...

background: previous studies have shown that some cytokines have protective effects on cartilage in joint diseases. in the current study, effects of il-4 against morphological changes and tissue degradation induced by il-1α on bovine nasal cartilage (bnc) explants were investigated. methods: fresh bnc samples were prepared from a slaughterhouse under sterile conditions. bnc explants culture was...

OBJECTIVE To determine if a new inhibitor, esculetin (EST), can block resorption of cartilage. METHODS Interleukin 1alpha (IL1alpha, 0.04-5 ng/ml) and oncostatin M (OSM, 0.4-50 ng/ml) were used to stimulate the release of proteoglycan and collagen from bovine nasal cartilage and human articular cartilage in explant culture. Proteoglycan and collagen loss were assessed by dimethylmethylene blu...

Dermatan sulfate proteoglycans were isolated from adult bovine sclera and adult bovine articular cartilage. Their immunological relationships were studied by enzyme-linked immunosorbent assays using polyclonal antibodies raised against the large and small dermatan sulfate proteoglycans from sclera and a polyclonal and monoclonal antibody directed against the small dermatan sulfate proteoglycans...

OBJECTIVE To investigate the single and combined effects of electromagnetic field (EMF) exposure and the insulin growth factor-I (IGF-I) on proteoglycan (PG) synthesis of bovine articular cartilage explants and chondrocytes cultured in monolayers. DESIGN Bovine articular cartilage explants and chondrocyte monolayers were exposed to EMF (75Hz; 1.5mT) for 24h in the absence and in the presence ...

INTRODUCTION Current therapies for articular cartilage defects fail to achieve qualitatively sufficient tissue regeneration, possibly because of a mismatch between the speed of cartilage rebuilding and the resorption of degradable implant polymers. The present study focused on the self-healing capacity of resident cartilage cells in conjunction with cell-free and biocompatible (but non-resorbab...

OBJECTIVE Articular cartilage is a complex tissue comprising phenotypically distinct zones. Research has identified the presence of a progenitor cell population in the surface zone of immature articular cartilage. The aim of the present study was to determine the in vivo plasticity of articular cartilage progenitor. DESIGN Chondropogenitor cells were isolated from bovine metacarpalphalangeal ...