Objective (s): The scarcity of articular cartilage defect to repair due to absence of blood vessels and tissue engineering is one of the promising approaches for cartilage regeneration. The objective of this study was to prepare an extracellular matrix derived decellularized bovine articular cartilage scaffold and investigate its interactions with seeded rat bone marrow mesenchymal stem cells (...

objective (s): the scarcity of articular cartilage defect to repair due to absence of blood vessels and tissue engineering is one of the promising approaches for cartilage regeneration. the objective of this study was to prepare an extracellular matrix derived decellularized bovine articular cartilage scaffold and investigate its interactions with seeded rat bone marrow mesenchymal stem cells (...

Background: This work aimed to show and compare the degradation time of some of cartilage extracellular matrix components using an in vitro model for cartilage degradation induced by interleukin-1 alpha. It is known that elucidation of molecular events under Interleukin-1 alpha induction of bovine nasal cartilage could obtain useful data to understand more about involving mechanisms for tissue ...

background: this work aimed to show and compare the degradation time of some of cartilage extracellular matrix components using an in vitro model for cartilage degradation induced by interleukin-1 alpha. it is known that elucidation of molecular events under interleukin-1 alpha induction of bovine nasal cartilage could obtain useful data to understand more about involving mechanisms for tissue ...

BACKGROUND The purpose of this study was to compare the frequency-dependent viscoelastic properties of human and bovine cartilage. METHODS Full-depth cartilage specimens were extracted from bovine and human femoral heads. Using dynamic mechanical analysis, the viscoelastic properties of eight bovine and six human specimens were measured over the frequency range 1 Hz to 88 Hz. Significant diff...

extracelllar matrix (ecm) materials from mammalian tissues have been broadly used for tissue engineering for animal models. while decellularization of tissues such as bladder, heart valve, knee meniscus and tendon has been performed, there are a few reports for articular cartilage. in this study we aimed to develop a technique to decellularize bovine articular cartilage as a biological construc...

Antibodies directed against whole bovine nasal-cartilage proteoglycan and against the hyaluronic acid-binding region and chondroitin sulphate peptides from the same molecule were used in immunodiffusion and immunoelectromigration experiments. Proteoglycans from bovine nasal and tracheal cartilage showed immunological identity, with all three antisera. Proteoglycans from pig hip articular cartil...

(1) Background: Mechanical loading is an essential part of the function and maintenance joint. Despite importance intermittent mechanical loading, this factor rarely considered in preclinical models cartilage, limiting their translatability. The aim study was to investigate effects dynamic compression on extracellular matrix during long-term culture bovine cartilage explants. (2) Methods: Bovin...

Monospecific antibodies to bovine nasal cartilage proteoglycan monomer and link protein were used to demonstrate that immunologically related molecules are present in the bovine eye and associated tissues. With immunofluorescence microscopy, reactions for both proteoglycan and link protein were observed in the sclera, the anterior uveal tract, and the endoneurium of the optic nerve of the centr...

Type IX collagen, a quantitatively minor collagenous component of cartilage, is known to be associated with and covalently cross-linked to type II collagen fibrils in chick and bovine cartilage. Type IX collagen molecules have also been shown to form covalent cross-links with each other in bovine cartilage. In the present study we demonstrate by structural analysis and location of cross-linking...