A shuttle cloning vector (pIL550) has been constructed which can be mobilized from Escherichia coli to Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus by complementation with the transfer functions of an IncP plasmid in trans, with a frequency of 10(-4) transconjugants per donor. We also present evidence for a DNA modification system in C. jejuni.

conclusions this study provides initial data on the prevalence and distribution of the flaa, cadf, ciab, cdtb, cgtb, docc and wlan genes in c. jejuni. background campylobacter jejuni (c. jejuni) is a major cause of human diarrheal disease. objectives this study was conducted to determine the prevalence of different pathogenic genes in isolates recovered from human stool samples in rosario, arge...

The aim of this study was to investigate the prevalence of virulence and Cytolethal Distending Toxin (CDT) genes in the Campylobacter isolates from intestinal contents and gall bladders of broilers and, to evalute their cytotoxic effects on HeLa cell cultures. These genes play important roles in bacterial adherence to intestinal mucosa, flagella-mediated motility, invasive capability and the ab...

The arylsulfatase gene from Campylobacter jejuni 81-176 encodes a predicted protein of 69,293 Da which shows no sequence similarity with other known arylsulfatases. The gene hybridizes to other Ast+ strains of C. jejuni and Campylobacter sputorum subsp. bubulus, as well as to many Ast- strains of C. jejuni.

Campylobacter jejuni and Campylobacter coli are common causes of gastroenteritis in humans. Infection with C. jejuni or C. coli is commonly acquired by eating undercooked chicken. The goal of this study was to develop specific detection assays for C. jejuni and C. coli isolates based on the cadF virulence gene and its product. The cadF gene from C. jejuni and C. coli encodes a 37-kDa outer memb...

Background: Campylobacter is a foodborne pathogen that is commonly associated with chicken. The aim of this work was to evaluate the prevalence of Campylobacter jejuni (as affected by refrigerated storage) in chicken samples obtained from the wholesale poultry market in the northern part of Riyadh City, Saudi Arabia. Findings: A gradual increase in the number of positive samples was noted durin...

DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C. jejuni. The assay is rapid and simple and can ...

We report the development of Campylobacter jejuni enteritis in a patient with preexisting humoral and cellular immune recognition of C. jejuni antigens. This is one of few studies in which the immunologic status of a person with regard to C. jejuni before and after C. jejuni infection is directly compared, and it is the only study of which we are aware that includes measurements of cellular imm...

Campylobacter jejuni and Campylobacter-specific bacteriophage were enumerated from broiler chicken ceca selected from 90 United Kingdom flocks (n = 205). C. jejuni counts in the presence of bacteriophage (mean log(10) 5.1 CFU/g) were associated with a significant (P < 0.001) reduction compared to samples with Campylobacter alone (mean log(10) 6.9 CFU/g).