background & aim: investigating the possibility of using medicinal plant’s seeds substitute for agar gel in plant tissue culture and determining the optimal practical conditions of them is the aim of this study. experimental: in order to study the new gelling agents for using in plant tissue culture, we designed a tow part experiment by means of the seeds of medicinal plants such as linum u...

Heart failure is one of the leading causes of death worldwide. End stage disease often requires heart transplantation, which is hampered by donor organ shortage. Tissue engineering represents a promising alternative approach for cardiac repair. For the generation of artificial heart muscle tissue several cell types, scaffold materials and bioreactor designs are under investigation. In this revi...

heart failure is one of the leading causes of death worldwide. end stage disease often requires heart transplantation, which is hampered by donor organ shortage. tissue engineering represents a promising alternative approach for cardiac repair. for the generation of artificial heart muscle tissue several cell types, scaffold materials and bioreactor designs are under investigation. in this revi...

The production of a functional cardiac tissue to be transplanted in the injured area of the infarcted myocardium represents a challenge for regenerative medicine. Most cell-based grafts are unviable because of inadequate perfusion; therefore, prevascularization might be a suitable approach for myocardial tissue engineering. To this aim, cells with a differentiation potential towards vascular an...

The myocardium of the embryonic chick heart, before it has been innervated, responds to concentrations of epinephrine as dilute as 1 part in 20,000,000 by an abrupt acceleration in its inherent rate of pulsation. The more slowly the heart beats initially, the more marked is the accelerating effect of epinephrine. Usually the initial acceleration is followed by a period during which the heart sl...

Peppers (Capsicum spp.) are recalcitrant to in vitro culture regeneration, making the application of vitro-based breeding strategies difficult. We evaluated impact different combinations auxins, cytokinins and micronutrients on induction direct organogenesis cotyledon hypocotyl explants C. annuum, baccatum chinense. found variation regeneration response among species type explant. In this way, ...

Prolactin (PRL) is a 23-kDa hormone produced by the pituitary and extrapituitary sites. The main target of PRL is the breast, where it affects cellular growth, differentiation, and milk production. Recent evidence suggests that locally produced PRL plays a role in breast tumorigenesis. Our objective was to examine PRL synthesis/release in different tissues of the human breast and determine the ...

Background: Most interactions between pathogenic microorganisms and their target host occur on mucosal surfaces of internal organs such as the intestine. In vitro organ culture (IVOC) provides an unique tool for studying host-pathogen interactions in a controlled environment. However, this technique requires a complex laboratory setup and specialized apparatus. In addition, issues arise when an...

As a prerequisite for exploring the mechanisms which lead to the formation and maintenance of the precise wiring patterns in the olfactory system, organotypic cultures of olfactory tissue from transgenic mice expressing green fluorescent protein (GFP) under control of the olfactory marker protein promotor have been established. Tissue specimen from embryonic stage 14 were explanted and kept in ...