We developed an enzyme immunoassay (ELISA) for quantitation of plasma cholinesterase substance concentrations in native plasma or serum samples. The ELISA assay is based on polyclonal (rabbit) antihuman cholinesterase and a highly specific monoclonal (mouse) antibody, with a commercially available peroxidase-conjugated (rabbit) antibody directed against mouse immunoglobulins as the signal carri...

People with genetic variants of cholinesterase respond abnormally to succinylcholine, experiencing substantial prolongation of muscle paralysis with apnea rather than the usual 2-6 min. The structure of usual cholinesterase has been determined including the complete amino acid and nucleotide sequence. This has allowed identification of altered amino acids and nucleotides. The variant most frequ...

Marcz M V S ~ ~L~,XNrS have been introduced during the past decade. Of all these relaxants, succmylcholine is the only drug with a fleeting action. This short duration of action has been ascribed to a rapid enzTmatlc destruction of succinylcholine in the body ( 1, 2). The only enzyme which is known to destroy succinylchohne m man ~s plasma eholinesterase. There are several synonyms for plasma c...

Frog skin cholinesterase is largely of the serum (pseudocholinesterase) type. For whole skin, the activity at 10 --1 ~ AcChC1 is 4.9/~l./mg. N/hr. Tela subcutanea isolated by dissection exhibits an activity of 65 #l./mg. N/hr. at 10 --j ~ AcChC1. Since about one-tenth of the nitrogen of the skin is located in the tela subcutanea, it is estimated that more than 90 per cent of the enzyme is assoc...

A screening aimed at obtaining a cholinesterase inhibitor of microbial origin was carried out using Pseudomonas butyrylcholinesterase. A mycelium-extract of a fungus strain, belonging to the genus Penicillium, was found to produce such an enzyme inhibitor. The inhibitor was purified and crystallized as colorless leaflets. From physical and chemical studies, the inhibitor was identified as being...

The original theory of neurqmuscular transmission involving acetylcholine led to the necessity to postulate an enzyme which hydrolysed it. This was done by Dale in 1914. It was not until 1932, however, that Stedman, Stedman, and Easson were able to prepare from horse serum an enzyme which could actually do this and which was called cholinesterase. The enzyme was then found to be present in a wi...

1512 CLINICAL CHEMISTRY, Vol. 25, No. 8, 1979 brano, T., Colorimetric determination of serum cholinesterase and its genetic variants by the propionylthiocholine-dithiobis(nitrobenzoic acid) procedure. Selected Methods Clin. Chem. 8,41-46 (1977). 5. King, J., Studies on human serum cholinesterases. Ph.D. thesis, University of Glasgow, 1974. 6. Garry, P. J., Serum cholinesterase variants: Examina...

A simplified manual and automated method is described for measuring serum cholinesterase activity and identifying the homozygous “usual,” heterozygous, and homozygous “atypical” forms of the enzyme. With butyrylthiocholine as substrate at pH 7.4, the activity is determined in two 0.05 mol/liter buffer systems, Tris and phosphate. The “usual” enzyme has the same activity in both buffer systems. ...

The additive is one component of paint that inhibits the growth bio-organisms in finished product with pesticide-like properties. Workers manufacture paints are populations at risk being exposed to heavy metals and pesticides, which enter workers through inhalation for a long time continuously. purpose this study was determine whether there relationship between age nutritional status Cholineste...