HE SERUM IRON DETERMINATION of Kingsley and Getchell (1) using the sensitive iron-complexing reagent, 4,7-diphenyl-1,10 phenanthroline (bathophenanthroline) appears to be useful in the clinical laboratory. However, the authors state that the procedure yields values significantly higher than the 2,2-dipyridyl reagent, but a satisfactory explanation for this difference is not readily apparent. In...

HE IMPORTANCE OF THE QUANTITATIVE ANALYSIS OF SERUM IRON has been long established (1, 2, 3), while the worthiness of the determination of serum copper is becoming of increasing interest (4, 5, 6). Therefore, it would be of real value if the concentration of both metabolically interrelated constituents of serum (7, 8, 9) could be determined accurately. It has recently been shown that copper and...

A sensitive determination method for mercury speciation analysis was developed. Four mercury species, mercury ion, methylmercury, ethylmercury, and phenylmercury, were complexed with emetine-dithiocarbamate (emetine-CS(2)), and then injected onto a HPLC instrument coupled with a tris(2,2'-bipyridine)ruthenium(III) chemiluminescence detection system. The emetine-CS(2) complexing agent was effe...

The biodegradability of four novel diethanolamine derivative complexing agents was examined by using two biodegradation tests standardised by OECD (301B and 301F). Ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA) were employed as reference substances. Biodegradation of the new complexing agents was studied both with unacclimated and acclimated inocula as well...

Botulinum toxin type A is a high molecular weight protein complex containing active neurotoxin and complexing proteins, the latter of which, it is believed, protect the neurotoxin when in the gastrointestinal tract, and may facilitate its absorption. Comparisons of conventional botulinum toxin type A drugs that include complexing proteins with the complexing protein-free formulation of Xeomin(®...

To the Editor: We read with interest a paper on phospholipid analysis in this journal (1) but deferred comment until the publication of our own paper on the subject, which we believe contains relevant material (2). Sandhu refers to the formation of a Prussian blue complex as the result of a reaction between phospholipids and a chromogenic reagent containing molybdenum. Clearly this cannot be so...

The distribution of adenosine deaminase and adenosine deaminase complexing protein in rabbit heart has been compared using immunohistochemical staining procedures. Sections (4-5 microns) of tissue fixed in Clarke's solution or paraformaldehyde and embedded in paraffin were stained by the peroxidase anti-peroxidase method for adenosine deaminase or complexing protein, using affinity purified ant...