A mitochondrial matrix copper ligand (CuL) complex, conserved in mammalian cells, is the likely source of copper for assembly of cytochrome c oxidase (CcO) and superoxide dismutase 1 (Sod1) within the intermembrane space (IMS) in yeast. Targeting the copper-binding proteins human Sod1 and Crs5 to the mitochondrial matrix results in growth impairment on non-fermentable medium caused by decreased...

In the present study, 2-pyridinecarboxaldehyde 2-pyridinecarboxylic acid hydrazone (PPAH) was prepared and its antitumor activity was evaluated. The inhibition of proliferation of PPAH against the HepG2 and HCT-116 cell lines was less effective, yet in the presence of copper ions, the mixture demonstrated excellent antitumor activity (IC50 at 2.75±0.30 µM for the HepG2 cell line, and 1.90±0.20 ...

The extraction and separation of copper(II), zinc(II), cobalt(II), and cadmium(II) were investigated. Both copper(II) and zinc(II) formed ammine-complexes, while cadmium(II) and cobalt(II) formed hydroxide precipitates in an ammonia medium. By the addition of sodium dodecylsulfate (SDS), a copper(II) complex formed an ion-pair (copper-ammine-DS), which was extracted into the SDS phase. However,...

A copper l immunoglobulin complex isolated from a myeloma patient with hypercupremia has been investigated in an attempt to localize and characterize the copper-binding site. Cleavage of the purified IgGl type immunoglobulin with papain yielded peptides which were identified as Fab and F, fragments by immunodiffusion. The fragments were separated by DEAE-cellulose chromatography and the Fab fra...

introduction sarcheshmeh copper mine is regarded as the second largest copper deposit in the world. this is one of the largest industrial-mining complexes in the world and the largest producer of copper in iran. this complex plays an important role in iran’s economy. in 2012, this complex created employment for about 8000 people in iran and produced 203065 tons of copper cathodes. however, high...

the relationship between different indices of the copper deficiency was studied in 136 slaughtered male cattle (< 2 years). copper status has been detected as marginally deficient in 57.4, 36.4, 34.6, and 28.2% of cattle based on the copper concentrations in plasma (5.5-14.5 μmol/l) and liver (19.7-189.1 mg/kgdm), plasma ceruloplasmin levels (85.4 - 178.2 mg/l) and erythrocyte superoxide dismut...

Redox active transition metal ions (e.g., iron and copper) have been implicated in the etiology of many oxidative stress-related diseases including also neurodegenerative disorders. Unbound copper can catalyze formation of reactive oxygen species (hydroxyl radicals) via Fenton reaction/Haber-Weiss chemistry and therefore, under physiological conditions, free copper is potentially toxic and very...