Development of techniques to isolate, culture, and transplant human spermatogonial stem cells (SSCs) has the future potential to treat male infertility. To maximize the efficiency of these techniques, methods for SSC cryopreservation need to be developed to bank SSCs for extended periods of time. Although, it has been demonstrated that SSCs can reinitiate spermatogenesis after freezing, optimal...

PURPOSE Cancer treatments for young women can permanently or temporarily affect fertility. The purpose of this retrospective analysis was to present the clinical experience and ethical considerations of fertility preservation in female oncology patients in a tertiary gynaecological department. METHODS Since 2002, in 37 patients fertility preservation was performed according to an institutiona...

In recent years, preservation of fertility in women has been of great importance, especially in patients exposed to deleterious conditions on fertility. Thus, cryopreservation of human gametes, embryos and ovarian tissue has become an essential part of assisted reproduction. This approach limits the number of embryos transferred, while supernumerary oocytes and/ or embryos can be used in subseq...

Common carp [Cyprinus carpio (C. carpio)] is an economically important fish species cultured mostly in Asia and Europe. Global production of farmed common carp was about 6.14% (3 172 488 tonnes) of the total world aquaculture production[1]. Artificial insemination can be applied to increase the production to meet this demand due to exploitation of natural stocks. Cryopreservation is a valuable ...

Background. Cryopreservation of spermatozoa with the best morphofunctional characteristics may allow their use for assisted reproductive technologies in goat breeding throughout year to effectively increase livestock. Aim. To evaluate influence seasons on morphological and morphometric fresh cryopreserved sperm. Methods. The ejaculate samples three sexually mature bucks Saanen breed were obtain...

Cryopreservation is becoming a very important tool for the long-term storage of plant genetic resources and efficient cryopreservation protocols have been developed for a large number of plant species. Practical procedures, developed using in vitro tissue culture, can be a simple and reliable preservation option of potato genetic resources rather than maintaining by vegetative propagation in ge...

BACKGROUND Although cord blood (CB) is a well-known source of hematopoietic stem cells, uncertainties exist regarding the quality of cryopreserved CB. We investigated the changes in quality of CB units according to the duration of cryopreservation. METHODS We analyzed CB units that were rejected from the Seoul Metropolitan Government Public Cord Blood Bank inventory after conventional process...

The clinical role of oocyte cryopreservation in assisted reproduction, as an adjunct to sperm and embryo cryopreservation, has been comparatively slow to evolve as a consequence of theoretical concerns related to efficacy and safety. Basic biological studies in the 1990's alleviated many of these concerns leading to more widespread adoption of the technology. While a number of babies were born ...

OBJECTIVE To evaluate the effect of vitrification and two other methods of slow cryopreservation on DNA integrity in expanded and nonexpanded blastocysts. DESIGN Prospective in vitro study. SETTING Tertiary care academic hospital. INTERVENTION(S) 1) Twenty-two expanded blastocysts (EB) and 17 nonexpanded blastocysts (NEB) vitrified in cryotips; 2) 15 EB and 16 NEB by slow freezing using p...