The present study was the first report on somatic embryogenesis of Tunisian chickpea (Cicer arietinum L.) particularly supposed to be recalcitrant and difficult manipulate in vitro. An efficient protocol has been developed for inducing indirect derived from immature zygotic embryo axis, young leaflet hypocotylar explants. Callogenesis achieved full-strength Murashige Skoog's (1962) (MS) basal m...

The present study demonstrates an efficient protocol for in vitro mass propagation of A. curassavica through direct as well as indirect regeneration. The effect of MS and L2 media supplemented with various concentrations and combinations of growth regulators has been studied. The growth regulators used include BAP and kinetin (cytokinins) and IAA, IBA, NAA and 2, 4-D (auxins). MS media proved b...

Shoot tip and nodal segment explants of Holarrhena antidysenterica when cultured on MS medium containing BAP (1.0-3.0 mg/l) with NAA (0.2-1.0 mg/l) and BAP (1.0-3.0 mg/l) with Kn. (0.2-1.0 mg/l) produced multiple shoots. Maximum multiple shoots was found in MS medium supplemented with BAP (2.0 mg/l) and NAA (0.5 mg/l). Subculture on the same medium resulted in rapid shoot multiplication at an a...

In vitro callogenesis was achieved from leaf explants of Boerhaavia diffusa Linn. on basal medium supplemented with different concentrations of BAP, NAA, Kn and IAA. Regeneration of shoot buds were observed on MS medium supplemented with BAP and NAA at different concentrations. In some of the replicates the shoot buds were elongated and terminated in flower buds. In vitro flowering occurred wit...

background and objectives: bitter melon is one of the anti-cancer medicinal plants that it’s wide level in vitro propagation and anti-cancer material production was necessary. different factors such as genotype, selected explants’ type and it`s preparation conditions influenced regeneration content. plant growth regulator`s effect on callogenesis and indirect regeneration from parangipetai indi...

The frequency of shoot regeneration and multiplication of P. harmala was influenced by the type of explant and kind and concentration of hormones. Of the various seedling explants, cotyledonary node exhibited maximum shoot regeneration frequency from axillary region on MS medium supplemented with 5 microM BAP. Addition of 0.1 microM NAA enhanced the efficacy of BAP for multiple shoot regenerati...

BACKGROUND Aloe vera L. is an important pharmaceutical plant from which several medicinal and cosmetic compounds are extracted. Aloe is naturally propagated through offset, which is a slow and expensive labor cost method with low economical income. OBJECTIVES In this study, the effect of different media on shoot proliferation of the shoot tip of Aloe vera L. was investigated. MATERIALS AND ...

Plants react differently to various media concentration and constituents. Growth of Senna alata (linn) inoculated on woody plant media (WPM) and Murashige & Skoog (MS) supplemented with growth regulators were investigated. Mature seeds were collected from the medicinal garden of National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan; latitude 70221N; longitude 30 551E. Seeds ...

Aponogeton ulvaceus Baker (Aponogetonaceae) is a commercially important ornamental aquatic plant species with traditional medicinal uses. Due to the low survival rate of seedlings, propagation by conventional means has been met with many difficulties. In this study, botanical aspects of A. ulvaceus were examined with regards to the morphology, anatomy and physiology of the plant and an efficien...