نتایج جستجو برای: direct sample pcr
تعداد نتایج: 971582 فیلتر نتایج به سال:
Aim: This study was done to evaluate polymerase chain reaction( PCR) and BACTEC culture in diagnosis of female genital tuberculosis. The findings of these diagnostic modalities were compared with that of direct microscopy and culture on Lowenstein-Jensen( LJ) medium and also with that of laparoscopy. Methods: Thirty clinically suspected cases of female genital tuberculosis were included in this...
Polymerase chain reaction (PCR) is a powerful molecular biological tool in the field of toxicity testing and diagnostics. The use of PCR for large-scale genetic testing requires an effective method of sample processing. Unfortunately, isolation of PCR-quality DNA is time-consuming. PCR performed directly on whole blood is preferred because of time efficiency, cost of the procedure, and possible...
The sensitivity, specificity and reproducibility of molecular genetic methods analysis largely depend on the quality preliminary preparation analyzed samples. During sample preparation, tasks disinfecting pathogenic material, lysing cell membranes, removing compounds impurities that inhibit polymerase chain reaction (PCR), as well concentrating nucleic acids are solved. purpose this work is to ...
Background: Protozoa have the ability to replace human lung. Over recent years, incidence of pulmonary infections caused by these microorganisms has increased, particularly in individuals with an immunodeficiency. The use appropriate diagnostic methods is important identification parasites secretions. Objectives: present study aimed evaluate and compare PCR-based gold standard method detect thr...
The aim of this study was to demonstrate the DNA of Paracoccidioides brasiliensis in human serum samples of patients with paracoccidioidomycosis using the polymerase chain reaction (PCR). The diagnosis of paracoccidioidomycosis (PCM) was defined by microscopic observation of the fungus on direct exam or histopathology, culture, and serological positivity. DNA from serum of 33 patients with PCM ...
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