Background: DNA vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. Bacteria of the genus Brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. Methods: To evaluate the use of a DNA immunization strategy for protection against brucellosis, a pla...

We describe the design, synthesis and biophysical characterization of a novel DNA construct in which a folded quadruplex structure is joined to a standard double helix. Circular dichroism, gel electrophoresis, three-dimensional UV melting and differential scanning calorimetry were all used to characterize the structure. Rigorous molecular dynamics simulations were used to build a plausible atom...

to construct of an eukaryotic expression vector encoding herpes simplex virus type 2 (hsv-2) glycoprotein d (gd2), an iranian isolate of hsv-2 was propagated in hela cell line and its dna was extracted and used as template in polymerase chain reactions (pcr), to amplify gd2 gene. primers were designed and the restriction enzyme sites for ecori and xhoi were considered at their 5′ ends respectiv...

Figure S1. Schematic illustration of puhA-zif268 DNA construct S2 Figure S2. Agarose gels showing amplification and assembly of the puhA-zif268 construct. S3 Figure S3 Sequence confirmation of puhA-zif268 construct by MWG Eurofins S3 Figure S4 SDS-PAGE and absorption spectra of purified WT and zfRC-LH1 S4 Figure S5 MALDI-TOF for the WT and zfRC-LH1 S5 Figure S6 MALDI-TOF mass spectra of DNA-cyc...

Ultraviolet radiation (UVR) is among major contributors to skin cancer in humans. Production of melanin serves as a main defense mechanisms against UVR damage. This pigment, which gives color hair, and eyes, protects the underlying tissue from However, some patient populations, this protection mechanism not effective, leaving patients highly susceptible cancer. Similarly, mycosporine like amino...

objective: the global hiv epidemic continues to expand and exceeding previous predictions. an effective vaccine represents the best hope to curtail the hiv epidemic. dna vaccines induce humoral and cellular responses and mimic live vaccines without their pathogenic potential. the importance of cd8+ ctl responses in controlling hiv and siv viremia has led to production of a series of vaccine can...