most of the systematic studies used in the development of human embryo culture media have been done first on mouse embryos. the general use of nmri outbred mice is a model for toxicology, teratology and pharmacology. nmri mouse embryo exhibit the two-cell block in vitro. the objective of this study was to evaluate and compare the effects of four kinds of culture media on the development of zygo...

  Effect of Cervical Mucus Aspiration with Insulin Syringe on Pregnancy Rate in ART Cycles     A. Aflatoonian MD1*, M. Asgharnia MD 2, N.Tabibnejad GP3     1- Associated Professor, Dept. of Gynecology, University of Medical Sciences and Research and Clinical Center of Infertility, Yazd, Iran   2- Assistant Professor, Dept. of Gynecology, Guilan University of Medical Sciences, Rasht, Iran   3- G...

The advent of human in vitro fertilization (IVF) over 30 years ago has made the oocyte and preimplantation embryo uniquely accessible. This accessibility has given rise to new micromanipulation techniques, such as intracytoplasmic sperm injection for treatment of male infertility, as well as embryo biopsy for preimplantation diagnosis of both genetic disease and aneuploidy, a major cause of ear...

Immunofluorescent studies were made on chicken kidney cell cultures infected with various temperature-sensitive (ts) mutants of an avian adenovirus, chicken embryo lethal orphan (CELO), which grow well at the nonrestrictive temperature of 31 degrees , but fail to grow at the restrictive temperature of 40 degrees . At 40 degrees some mutants (ts 5, 8, and 10) accumulated viral antigen in the cyt...

Previous results with thyroid secretory cells in monolayer culture seem contradictory with respect to phenotypic stability of this cell type. On the one hand, in "minimal" medium the cells lose structural and functional specializations which can be returned only by three-dimensional growth in organ culture upon addition of fibroblasts derived from the thyroid capsule. On the other hand, in "ric...

Formation of neuromuscular junctions in embryonic cultures has been reviewed. Anatomical and electrophysiological evidence has been presented which demonstrates the formation de novo of synaptic contacts between nerve and muscle in culture; in (1) organ cultures of spinal cord explanted intact with attached myotomes, (2) organ cultures of in vitro coupled explants of spinal cord and skeletal mu...

Argos is a secreted molecule with an atypical EGF motif. It was recently shown to function as an inhibitor of the signaling triggered by the Drosophila EGF receptor (DER). In this work, we determine the contribution of Argos to the establishment of cell fates in the embryonic ventral ectoderm. Graded activation of DER is essential for patterning the ventral ectoderm. argos mutant embryos show e...

An overview is given on selected cost-drivers within an assisted reproduction technology (ART) laboratory, such as procedural costs; sperm preparations; laboratory supplies including embryo culture media and cryopreservation. Depending on the nature of an ART unit, i.e. private vs. public/tertiary, the structure of the unit will differ with regards to costs, services offered, and general patien...

In previous studies, prostaglandins have been applied to organ cultures of dorsal skin from the chick embryo (11, 12) . When prostaglandin-B, (PGB,) is added to the culture medium in which skin pieces are explanted at a time when feather organ loci are present, the latter fail to develop during incubation but the skin continues to grow and mature . Control skins demonstrate normal feather devel...

Intercellular invasion is the active migration of cells on one type into the interiors of tissues composed of cells of dissimilar cell types. Contact paralysis of locomotion is the cessation of forward extension of the pseudopods of a cell as a result of its collision with another cell. One hypothesis to account for intercellular invasion proposes that a necessary condition for a cell type to b...