نتایج جستجو برای: embryos

تعداد نتایج: 43680  

اسماعیل نژادمقدم , امیر, بحرالعلومی , سمیه, طالب پور , فرشته, کریم پورملک شاه , عباس علی, کلته , آیلر,

Background and purpose: The development of pre-implantation mammalian embryos in vitro is compromised, compared with those grown in vivo. Selecting embryos with a high implantation potential is one of the most important challenges in the field of assisted reproductive technology. The aim of this study was to postulate morphometrical characteristics of good quality embryos, with comparisons be...

Hans-Christoph Duba Manuela Puchner Maria Maurer, Omar Shebl Peter Oppelt Richard Bernhard Mayer Thomas Ebner

Background Selecting the best embryo for transfer, with the highest chance of achieving a vital pregnancy, is a major goal in current in vitro fertilization (IVF) technology. The high rate of embryonic developmental arrest during IVF treatment is one of the limitations in achieving this goal. Chromosomal abnormalities are possibly linked with chromosomal arrest and selection against abnormal fe...

Ahmad Hoseini, Hossein Mazdarani, Mojtaba Rezuadeh, Noredin Ne'amatollahi,

  SUMMARY To study the effect of vero cells on preimplantation mouse embryos two groups of experiment were carried out. In experiment 1, pronuclear stage mouse embryos were co-cultured with Vero cells in RPMI medium and were examined under microscope for 4 days with 24h interval. The results showed co-cultured group developed better compared to control (87% to 80%, P<0.05) although, the rate ...

H. Pahang M.A Kiani S.A.M Nematollahi Mahani S.N Nematollahi Mahani

Introduction: Mammalian embryos as well as oocytes are prone to various doses of visible light during manipulations in laboratory. The present study was designed to investigate the effects of visible light on the development of mouse 2- cell embryos. Method: Non-pregnant female NMRI mice were super-ovulated with i.p. injection of 7.5 iu PMSG followed 48 hours later with 10 iu hCG. Forty eight h...

Majidi Gharenaz N, Mazahei Z Movahedin M

Background: The aim of this study was evaluation of developmental rate and Bax, Bcl-2 and ErbB4 genes expression following re-vitrification in compact and early blastocysts stages. Materials and Methods: 5-8 cell embryos were collected from female mature mice, 60-62 hours post hCG injection. The embryos were divided to five groups including: fresh, vitrified at 5-8 cells, vitrified at blastocys...

Azam Agha-Rahimi Mohammad Ali Khalili, Morteza Anvari

Background The purpose was to investigate the effect of the duration of gamete incubation on fertilization rate, embryo cleavage, and embryo quality before and after freezing in mice. MaterialsAndMethods Ovulated oocytes collected from superovulated mice after ip injection of PMSG and hCG were divided randomly into control and experimental groups. Oocytes from the control group were inseminated...

Journal: :international journal of reproductive biomedicine 0
nasrin majidi gharenaz mansoureh movahedin zohreh mazaheri shahram pour beiranvand

background: nowadays, oocytes and embryos vitrification has become a routine technique. based on clinical judgment, re-vitrification maybe required. but little is known about re-vitrification impact on genes expression. objective: the impact of re-vitrification on apoptotic and implanting genes, bax, bcl-2 and erbb4, at compaction stage embryos were evaluated in this study. materials and method...

A. Niasari-Naslaji, E. Naghzali F. Barati, F. Sarhaddi K. Razavi M. Bolourchi

During spring, summer and winter seasons, Sistani donor cows, with normal reproductive status, were superovulated and embryos were recovered non-surgically on day 7. Grade A blastocyst embryos were either transferred fresh (spring) or frozen (summer and winter). Recovered embryos during summer and winter were exposed to glycerol and frozen using conventional method. During spring season, recipi...

قندی, نسیبه, اسماعیل نژاد مقدم, امیر, کریم پور ملکشاه , عباسعلی,

Background and purpose: To assess the effect of equilibration time on the vitrified-warmed two cell mouse embryos. Material and Methods: Two cell embryos were obtained from oviducts of super ovulated female NMRI mice. The embryos were randomly allocated in four groups and vitrified using cryotop and two-step mediaprotocol. The experimental groups were: 1) embryos were equilibrated in vitrif...

Journal: :journal of agricultural science and technology 2014
l. l. song h. n. zhang h. q. zhao y. l. jiang m. f. hou

taxus chinensis var. mairei is a rare and endangered medicinal plant species distributed in china. in order to promote fast propagation and preserve the natural resources, conditions for in vitro germination and seedling development of embryos of t. chinensis var. mairei from anhui or zhejiang were investigated. results showed that in vitro germination rate of excised embryos cultured under 14 ...

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