ANALYSIS OF NON-ESTERIFIED FATTY ACIDS IN HUMAN PLASMA BY CAPILLARY GAS-CHROMATOGRAPHY WITH SPLITLESS INJECTION. The aim of the present work was to test the combination of non-esterified fatty acid (NEFA) isolation using fumed silicon dioxide with capillary gas-chromatography (C-GC) with splitless injection for the analysis of NEFAs in human plasma. Injection volume, solvent re-condensation and...

Inter-esterification is one of the processes used to modify the physico-chemical characteristics of oils and fats. Inter-esterification is an acyl-rearrangement reaction on the glycerol molecule. On the other hand, hydrogenation involves addition of hydrogen to the double bonds of unsaturated fatty acids. Due to health implications of trans fatty acids, which are formed during hydrogenation, th...

The metabolism of (14)C-labeled fatty acids and triacylglycerols was followed in intact germinating oil palm seedlings as well as in tissue slices. In the germinating seedling, the shoot contained a normal pattern of membrane fatty acids (mainly C(16), C(18:1), C(18:2)) but the kernel contained about 68% C(12) and C(14) fatty acids. Haustorium fatty acids were intermediate between the two. [(14...

The development of atherosclerosis has been studied in White Carneau and Show Racer pigeons. The results of analytical studies indicate that the disease process is characterized by the accumulation of various lipids, and especially of sterol esters and free sterols. By perfusion of arteries with blood serum or with tissue culture medium containing (214labeled acetate, significant synthesis of l...

The use of marine n-3 polyunsaturated fatty acids (n-3 PUFA) as supplements has prompted the development of concentrated formulations to overcome compliance problems. The present study compares three concentrated preparations - ethyl esters, free fatty acids and re-esterified triglycerides - with placebo oil in a double-blinded design, and with fish body oil and cod liver oil in single-blinded ...

A hitherto unreported esterified form of steryl glucoside has been isolated from potato tuber lipids and soybean phosphatides. It is composed of sterol, glucose, and fatty acid in the molar ratio 1 : 1 : 1. Four components of the sterol moiety have been detected ; they include &sitosterol and stigmasterol (identified by gas-liquid chromatography). Fatty acids are palmitic, stearic, oleic, linol...

The relationship between intestinal bile acids and the micellar solubilization of lipids was studied by determining micellar bile acids and distribution of cholesterol and 0-sitosterol, their esters, and of hydrolysis products of triglycerides (labeled with H-tristearate), free fatty acids, monoglycerides and diglycerides between the micellar and oil phases of the intestinal contents for 1% h a...

Lipid composition of the fat body and haemolymph of male milkweed bug, On.cc@eltus fasciatus, was determined. Triglycerides were the predominant lipids of the fat body while diglycerides accounted for the major lipid in the haemolymph. Sterols, sterol esters, and non-esterified fatty acids were present in both fat body and haemoIymph besides triglycerides and diglycerides. Only traces of monogl...

The physiological response to starvation involves increased muscle proteolysis and adipose tissue lipolysis that supply amino acids and non-esterified fatty acids ('free fatty acids') for gluconeogenesis, oxidation and ketogenesis. In the present issue of Clinical Science, Moller and co-workers show that, in humans, IHL (intrahepatic lipid) content, measured using (1)H-magnetic resonance spectr...