The aim of this study was to assess the influence of selected cardiovascular and antidiabetic drugs on in vitro pepsin activity. A total of 13 drugs were analyzed at one concentration (one tablet of drug in 50 mL of solution with deionized water). The enzyme activity was determined by the Folin method based on the reaction of tyrosine with the Folin reagent. It was found that ACE inhibitors (ap...

The He-Ne laser (632.8 nm) and the concept of optothermal window (OW), a variant of the open photoacoustic cell, were combined with the Folin-Ciocalteu colorimetry assay to quantitate phenolics in four red wines. The total polyphenolic content in selected red wines varied between 786 and 1630 mg/L gallic acid equivalent (GAE) as determined by OW-Folin-Ciocalteu colorimetry, which compares well ...

The objective of this study was to investigate the influence of the concentration of Al ions and the substrate/enzyme ratio on pepsin and trypsin activity in vitro. The experimental design was a combination of three Al ion concentrations (0.25; 2.5 and 25.0 μg Al/ml of reaction solution) and two substrate/enzyme ratios (S/E = 10 and 100 for pepsin, and 100 and 1000 for trypsin). Enzymatic activ...

R. G. Canti [1916] bas used the hypobromite method for obtaining approximate values for the amount of urea in the cerebro-spinal fluid of patients suffering from a variety of diseases. Being desirous of controlling this method by others more reliable he asked R. L. M. Wallis to determine the urea by the urease method in some cases, and G. Graham by the Folin method in others, and the writer to ...

Of the existing methods for the determination of amino acid nitrogen in animal tissues, those of Folin and Denis (1) and of Van Slyke (2) are best known and most extensively employed. Both of them are long and tedious and call for rather large tissue samples. Also methyl alcohol containing zinc chloride is employed as an extractive by Folin and Denis and ethyl alcohol as a protein precipitant b...

Although a number of methods have been devised for the quantitative estimation of acetone in the urine, at the present time the method of Messinger,2 as modified by Huppert,s is the one most frequently employed, and is usually regarded as the most accurate one at our command.4 A recent paper by Folin5 offers the first satisfactory method for separately determining the acetone and diacetic acid ...

Although a number of methods have been devised for the quantitative estimation of acetone in the urine, at the present time the method of Messinger,2 as modified by Huppert,s is the one most frequently employed, and is usually regarded as the most accurate one at our command.4 A recent paper by Folin5 offers the first satisfactory method for separately determining the acetone and diacetic acid ...

The search for a simple, cheap, and reliable method of serum uric acid estimation has continued ever since the classic thread test of Garrod (1854); for many years Folin's phosphotungstic acid method and its numerous modifications have been employed (Folin, 1930; Brown, 1945; Caraway, 1955; Beale, 1954; Henry, Sobel, and Kim, 1957; Archibald, 1957). While the technique is easy, the method is ne...

Antioxidant potential (AOP) is not only the property of the matrix analyzed but also depends greatly on the methodology used. The chromogenic radicals 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and Folin-Ciocalteu (FC) assay were applied to estimate how the method and the composition of the assay solvent influence the AOP determined fo...