infectious keratoconjunctivitis (ikc) is a painful, highly contagious ocular disease in sheep and goats. this study was carried out for identification and characterization of causative agent of ocular disease in a sheep flock consisting of 300 ewes in mashhad, iran. several ocular swabs were taken from affected animals. the samples were pooled and processed in a laboratory for isolation of susp...

A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T-DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The technique has been used successfully for the isolation of the maize Bx1 gene tagged by a Mutator el...

Although, many genes are involved in pathogenesis of Campylobacter, racR gene considered the main gene for Campylobacter pathogenicity in humans. The purpose of this study was determined the prevalence of recR gene in Campylobacter spp. isolated from domestic animals and water. To perform the study 392 fecal and water samples were collected from poultry(182), cow(141), sheep and goat(41) and w...

Infectious keratoconjunctivitis (IKC) is a painful, highly contagious ocular disease in sheep and goats. This study was carried out for identification and characterization of causative agent of ocular disease in a sheep flock consisting of 300 ewes in Mashhad, Iran. Several ocular swabs were taken from affected animals. The samples were pooled and processed in a laboratory for isolation of susp...

Ecological speciation is considered an adaptive response to selection for local adaptation. However, besides suitable ecological conditions, the process requires assortative mating to protect the nascent species from homogenization by gene flow. By means of a simple model, we demonstrate that disruptive ecological selection favors the evolution of sexual preferences for ornaments that signal lo...

In this research, processed or low samples containing corn products (corn semolina, flour, etc.) and soybean were randomly collected from the market, 25 in total (chips, nuts, cereals, flour) analyzed for genetic modification using DNA based detection method, polymerase chain reaction. First, homogenization of was performed. Then isolation done by Cetyl Trimethyl Ammonium Bromide (CTAB) Roche H...

Background: Adipose tissue is a main source for isolation of equine mesenchymal stem cells (MSCs) at different ages. It seems that characteristics of adipose-derived MSCs especially gene expression profile are changing along with age increase. A proper reference gene is required for normalizing data in gene expression analysis by qRT-PCR. This study aimed to evaluate whether GAPDH has a stable ...

lipases catalyze hydrolysis and synthesis of triacylglycerols. these enzymes,especially the microbial ones, have wide commercial and industrial usage. bacillus subtilis lipase has interesting properties such as small size and tolerance to basic ph; therefore, developing techniques for its recognition and isolation is the focus of research in many laboratories. in the present study, two factors ...