abstract in order to study of two hormones effects of gibberellin and benzyl adenine on (zantedeschia aethiopica cv.childsiana) pot plant characteristics, non dormant rhizomes dipped preplanting in gibberellin solution with concentrations including (0, 100, 200 and 500 ppm) and sprayed in two weeks once to flowering time with benzyl adenine solutions including (0, 100, 200 and 500 ppm). interac...

Gibberellins are phytohormones that regulate growth and development of plants. Gibberellin homeostasis is maintained by feedback regulation of gibberellin metabolism genes. To understand this regulation, we manipulated the gibberellin pathway in tobacco and studied its effects on the morphological phenotype, gibberellin levels and the expression of endogenous gibberellin metabolism genes. The o...

The development of seed dormancy is an aspect of seed maturation, the last stage of seed development. To isolate mutants of Arabidopsis thaliana that are affected in this process, we selected directly for the absence of dormancy among freshly harvested M2 seeds. The screen yielded two mutants exhibiting a reduced dormancy, rdo1 and rdo2, that are specifically affected in dormancy determined by ...

Gibberellin A(1)-3,4-(3)H was prepared by selective catalytic reduction of gibberellic acid with a mixture of tritium and hydrogen. (3)H-GA(1) was applied at physiological concentrations to dwarf peas and the metabolism of the hormone was investigated. (3)H-GA(1) was converted to an acidic, biologically active compound. Radioactive but biologically inactive compounds were also found in the neut...

This study was undertaken to elucidate the dominant endogenous gibberellins (GA) and the changes in their levels in germinating barley during malting. Also, a method for determining gibberellins in barley and malt by combined gas chromatography-mass spectrometry (GC-MS) was developed. Endogenous gibberellins in germinating Fuji Nijo I I and Betzes barley were sequentially extracted with ethanol...

Ras-related guanosine triphosphate (GTP)-binding nuclear protein (Ran) GTPases function as molecular switches and regulate diverse cellular events in eukaryotes. Our previous work suggested that DlRan3B is active during longan (Dimocarpus longan Lour.) somatic embryogenesis (SE) processes. Herein, subcellular localization of DlRan3B was found to be localized in the nucleus and expression profil...

The isolation, crystallization, and partial identification of potato factor II, a stimulator from the chemically neutral fraction of potato extract, is described. The compound was originally found to stimulate elongation of dwarf peas grown under red light, a gibberellin bioassay. It melts between 137 degrees and 139 degrees . In paper chromatography it migrates to R(F) 0.62 in isopropyl alcoho...

Radioactive gibberellin A(5) ((3)H-GA(5)) was synthesized from gibberellic acid. When it was applied to dwarf peas grown in the dark, an average of 3% was converted to another acid gibberellin within 48 hours. The biological activity of the metabolite did not account for the response to applied GA(5). GA(5) is therefore assumed to be biologically active per se.(3)H-GA(5) did not appear to form ...

A homogenate from dwarf pea seedlings that had been treated with [(3)H]gibberellin A(1) was separated by Sephadex G-200 into two protein fractions, of high and intermediate molecular weight, with affinity for [(3)H]gibberellin A(1). Neither fraction bound [(3)H]gibberellin A(8), the only metabolite of [(3)H]gibberellin A(1) detected in significant quantity in extracts of pea seedlings. These pr...