Here, we describe a simple and sensitive method that allows fluorescent detection of glycoproteins on polyvinylidene difluoride (PVDF) membrane. We used periodic acid oxidation of carbohydrate chains of glycoproteins and fluorescent labeling with 8-aminonaphthalene-1,3,6-trisulfonate (AN-TS) by reductive amination. We developed an additional method to enhance the ability of PVDF to absorb glyco...

Objective: Coronaviruses and paramyxoviruses, both of which are RNA viruses, have similar structural glycoprotein characteristics. It is considered that antibodies developed against these structures may protect by cross-reaction because the glycoproteins viruses related to their ability infect cell.

Glycoproteins are involved in various significant biological processes and have critical functions physiology pathology by regulating activities molecular signaling pathways. The variety of enzymes used protein glycosylation the wide range diversity resulting glycoproteins pose a challenging task when attempting to simulate these silico. This study aimed establish define necessary structures pr...

Sindbis virus was iodinated by using the enzyme lactoperoxidase, an iodination technique which labels only surface proteins. By this technique, the two viral glycoproteins are labeled, and the internal viral protein is not. The two glycoproteins are iodinated to strikingly different extents. This difference in susceptibility to iodination apparently is due to the position or conformation of the...

S-Gene-associated glycoproteins (S-glycoproteins) from styles of Nicotiana alata, identified by non-equilibrium two-dimensional electrophoresis, were purified by cation exchange fast protein liquid chromatography with yields of 0.5 to 8 micrograms of protein per style, depending on the S-genotype of the plant. The method relies on the highly basic nature of the S-glycoproteins. The elution prof...

Glycoproteins were isolated from the blood sera of the Antarctic fishes Tremafomus borchgrevinki and Dissostichus mawsoni. Some of these glycoproteins have the unique property of depressing the freezing point of water more than expected on the basis of the number of particles present in solution and are termed active glycoproteins. These freezing point-depressing glycoproteins depress the freez...

We recently reported (Pereira et al., Proc. Natl. Acad. Sci. U.S.A. 78:5202-5206, 1981) that herpes simplex virus 1 and 2 glycoproteins, previously designated gA and gB, could not be differentiated by a bank of independently derived type-specific and type-common monoclonal antibodies. We also reported that from lysates of infected Vero cells, all but one monoclonal antibody precipitated gA/B gl...

Tritiated borohydride reduction of cells treated in situ with galactose oxidase and neuraminidase was used to label externally oriented glycoproteins and glycolipids of the plasma membrane. The tritium was incorporated into galactose and galactosamine residues of both glycoproteins and glycolipids of two rat hepatoma cell types-monolayer H-35 cells and suspension cultures of HTC cells. The glyc...

Glycoproteins perform extra- and intracellular functions in innate and adaptive immunity by lectin-based interactions to exposed glyco-determinants. Herein, we document and mechanistically explain the formation of subcellular-specific N-glycosylation determinants on glycoproteins trafficking through the shared biosynthetic machinery of human cells. LC-MS/MS-based quantitative glycomics showed t...