Four cDNAs for spermidine synthase (SPDS), which converts the diamine putrescine to the higher polyamine spermidine using decarboxylated S-adenosylmethionine as the co-factor, were isolated from Nicotiana sylvestris, Hyoscyamus niger, and Arabidopsis thaliana. When the N.sylvestris SPDS cDNA was expressed in a SPDS-deficient E. coli mutant, the recombinant protein showed high SPDS activity, but...

the genus hyoscyamus belongs to the tribe hyoscyameae miers of solanaceae family. variation in protein bands elaborates the relationship among the collections from various geographical regions. in this study the seed storage protein diversity of 19 accessions of hyoscyamus (h. niger, h. reticulatus and  h. pusillus) from west azerbaijan (iran) was investigated according to the sodium dodecyl su...

The genus Hyoscyamus belongs to the tribe Hyoscyameae Miers of Solanaceae family. Variation in protein bands elaborates the relationship among the collections from various geographical regions. In this study the seed storage protein diversity of 19 accessions of Hyoscyamus (H. niger, H. reticulatus and  H. pusillus) from West Azerbaijan (Iran) was investigate...

Various plant organ segments including segments from roots (root tip, and root collar), stems, leaves, and also seeds of several Hyoscyamus species (i.e. H. pusillus, H. arachnoideus, annual and biennial H. niger from two distinct geographical regions) were cultured on MS media with various growth regulator combinations, to assess the ability of flower production. Statistical assessments showed...

Hyoscyamine is epoxidized to scopolamine via 6beta-hydroxyhyoscyamine in several solanaceous plants. 6,7-Dehydrohyoscyamine has been proposed to be an intermediate in the conversion of 6beta-hydroxyhyoscyamine to scopolamine on the basis of the observation that this unsaturated alkaloid is converted to scopolamine when fed to a Datura scion. To determine whether a dehydration step is involved i...

numerical taxonomy and seed storage protein analysis of hyoscyamus species of iran was carried out with the aim to illustrate species inter-relationship and to check the sub-generic taxonomic treatment proposed for the genus. cluster analysis of morphological and protein data grouped the species in three separate clusters which supports the relationships of h. niger with h. reticulatus and h. k...