Glyceraldehyde 3-phosphate dehydrogenase (D-glyceraldehyde-3-phoshate:nicotinamide adenine dinucleotide oxidoreductase (phosphorylating), EC 1.2.1.12) forms a complex with 3-pyridinealdehyde-NAD which survives precipitation with 7% perchloric acid. The molar ratio bound 3-pyridinealdehyde-NAD to the enzyme is 2.5 to 2.9. Lactate, malate, and alcohol dehydrogenases do not form acid-precipitable ...

enzyme is a true cathepsin L. No enzyme with properties of cathepsin B were detected in T. cnrzi, although the enzyme described by Cazzulo and co-workers [3-51 has some features in common with cathepsin B. The enzyme that cleaves Ala-Ala-pNA is inhibited by E64, iodoacetic acid and leupeptin, but is not affected by inhibitors of serine, metalloor aspartic peptidases. It does not release p-nitro...

Manganese dioxide (MnO2) is a Mn deposit widely accumulated in the corrosion layer of pipelines, and iodide (I−) halogen ion frequently detected waters. The biofilm dwelling on scales often secretes extracellular polymeric substances (EPS) into drinking water. paper aimed to study I− oxidation by MnO2 iodinated disinfection byproducts (I-DBPs) formation with EPS as precursor. More than 93% form...

Plasmodium falciparum triose-phosphate isomerase, a homodimeric enzyme, contains four cysteine residues at positions 13, 126, 196, and 217 per subunit. Among these, Cys-13 is present at the dimer interface and is replaced by methionine in the corresponding human enzyme. We have investigated the effect of sulfhydryl labeling on the parasite enzyme, with a view toward developing selective covalen...

The reaction of iodoacetic acid with ribonuclease has been studied recently by Gundlach et al. (1). Inactivation of the enzyme occurred over a wide range of pH. The authors concluded that at acid pH the reaction involved solely methionyl residues, that at pH 5.5 a histidyl residue was the principal site of attack, and that at alkaline pH values significant reaction at lysyl residues occurred. O...

The half-cystine residues involved in linking the acidic and basic polypeptides were determined for several glycinin subunits. The cystines were localized with specific cyanogen bromide fragments either by comparing the electrophoretic mobility of nonreduced and reduced fragments, or by co-purifying and then determining the NH2-terminal sequence of the covalently linked fragments. Residues invo...

The effects of various metabolic inhibitors on the time course of changes in membrane potential was studied using intracellular recordings from CA1 hippocampal neurons in vitro. Concurrent application of cyanide and iodoacetic acid, agents which block oxidative phosphorylation and glycolysis respectively, result in more rapid loss of membrane function than blockade of either pathway alone. This...