The reaction between iodoacetic acid and sulfhydryl compounds has received much attention in recent years. Dickens (1) has shown that this reaction proceeds with the formation of HI and the substitution of a carboxymethyl group for the hydrogen of the thiol group. Smythe (2) has measured the rate of reaction of iodoacetic acid with various sulfhydryl compounds by estimation of the carbon dioxid...

In studying the reaction which takes place between glycine and iodoacetic acid in certain alkaline buffer solutions (1), it was necessary to consider the saponification of iodoacetic acid by hydroxyl ions. This occurs simultaneously as an independent side reaction. By determining the velocity constants for the saponification in the solutions in which glycine was omitted, the rate of the iodoace...

A covalent, catalytic intermediate of cytosolic liver acetyl coenzyme A: arylamine N-acetyltransferase (EC 2.3.1.5) from rapid acetylator rabbits (III/J) was isolated and chemically characterized. The active site was further studied using two covalent inhibitors, [2-3H]iodoacetic acid and bromoacetanilide. Inhibition experiments with [2-3H]iodoacetic acid at pH 6.9 showed that the incorporation...

The discovery by Lundsgaard (1) of the inhibitory effect of iodoacetic acid on tissue glycolysis and yeast fermentation has aroused interest in the possibility of its use in controlling the abnormally high glycolytic rate of the cancer cell. A knowledge of its mode of action is essential to progress in this direction. Lundsgaard believes that the iodoacetic acid acts by preventing the formation...

In investigating the respiratory metabolism of cells whose growth has been inhibited by the use of various agents, it is often advantageous for the action of the inhibitor to be irreversible. Following an exposure to an inhibitory agent of this type, the cells may be freed from the medium containing the inhibitor, thus permitting an investigation of the altered metabolism under a variety of env...