We describe the kinetics of pentoxifylline formation from lisofylline in human liver microsomes using selective inhibitors of cytochrome P450 isozymes, correlation studies with specific isozyme activities, and cDNA-expressed human CYP1A2 and 2E1. A biphasic model fitted the data best for the formation of pentoxifylline, Km1 = 0.282 +/- 0.135 microM, Vmax1 = 0.003 +/- 0.001 nmol/min/mg protein, ...

Abstract The present work focusses on development of a safe, inexpensive, and more accessible source for biosynthesis silver nanoparticles. Four different in-house probiotic isolates, i.e., Lactobacillus pentosus S6, plantarum F22, crustorum F11, paraplantarum KM1 isolated from food sources, were used in the current study to check their ability synthesize All probiotic-synthesized nanoparticles...

1. The enzyme kinetics of dextromethorphan O-demethylation in liver microsomes from three extensive metabolisers (EM) with respect to CYP2D6 indicated high (Km1 2.2-9.4 microM) and low (Km2 55.5-307.3 microM) affinity sites whereas microsomes from two poor metabolisers (PM) indicated a single site (Km 560 and 157 microM). Similar differences were shown for 3-methoxymorphinan O-demethylation to ...

We have examined the binding of [3H]bradykinin to bovine myometrial membranes and assessed its sensitivity to guanine nucleotides. Total binding displayed a typical B2 kinin receptor specificity. However, saturation binding isotherms were resolved into at least two components with Ko values of 8 pM (45%) and 378 PM (55%). Low affinity binding exhibited relatively rapid rates of association (12,...

In the current state of huge waste production and energy crisis, there is a need to find additional alternate resources options for management. The present study was designed measure potential different fruit wastes serve as substrate lipid accumulation in oleaginous bacteria. For this purpose, three novel bacterial strains (AF3, KM1 KM10) isolated from crude oil samples were systematically com...

Worldwide NH3 production reached 0.18 Gton in 2019, and 1–2% of the global CO2 emissions are due to large-scale synthesis (1 billion tons / year). A catalyst for ammonia has been obtained by pyrolysis iron phthalocyanine (FePc) precursor under N2, followed impregnation with alkali metals (Na, Li, K, Cs) H2 treatment. Characterization (XPS, XRD, HR-TEM, ICP-OES, TGA, CHNS analysis, BET) revealed...

The hormone-stimulated 'dense-vesicle' cyclic AMP phosphodiesterase was solubilized as a proteolytically 'clipped' species, and purified to apparent homogeneity from rat liver with a 2000-3000-fold purification and a 13-18% yield. It appeared to be a dimer (Mr 112,000), of two Mr-57,000 subunits. Solubilization of either a liver or a hepatocyte membrane fraction, with sodium cholate in the pres...