results we found 28 cases with positive results for b. melitensis by multiplex pcr technique which was significantly different from of sat (p<0.05). six samples were positive for b. abortus by pcr. conclusion the results of present study showed that multiplex pcr assay is a rapid and sensitive technique for diagnosis of brucellosis compared to sat. however it is more accurate when coupled with ...

conclusions the present findings reveal a relatively high presence of anti-hev iggs and clearly indicate that hev infection is still present in northern algeria. further, the prediction of hev genotype using different antigens generated from the different hev genotypes shows that the causative strains are more likely to be of genotype 1. background two hepatitis e virus (hev) outbreaks occurred...

background rheumatoid arthritis (ra) is the most common chronic inflammatory disease of unknown etiology. in this regard, the role of bacterial superantigens (as an effective agent) were considered. objectives this study aimed to assess staphylococcal enterotoxin e in the blood of patients with rheumatoid arthritis. patients and methods a total of 83 blood samples of patients with ra were studi...

An enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of Bacteroides with the EDTA-released outer-membrane complex from 29 characterized strains of Bacteroides species. With only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the het...

background anaerobic-inducible promoters are alternatives of chemical-inducible promoters for expression of recombinant proteins especially in conditions where chemical induction is not possible or anaerobic conditions are preferable. the nirb promoter is the promoter of the first gene of nir operon in escherichia coli, which encodes nadh-dependent nitrite reductase. this promoter is naturally ...