conclusions our results suggest that human exposure to mcpyv occurs throughout life. the mcpyv antibody levels remained high among older adults in our population, consistent with reports from other populations. patients and methods in this cross-sectional study, we collected 440 serum samples from healthy individuals 2 to 78 years of age who visited the pasteur institute’s clinic in tehran, ira...

In the Trial to Reduce Alloimmunization to Platelets (TRAP) study, 101 of 530 participants became refractory to platelet transfusions without evidence of HLA or human platelet antigen (HPA) antibodies. We used a more sensitive bead-based assay to detect and quantify HLA antibodies and a qualitative solid-phase enzyme-linked immunosorbet assay for HPA to determine whether low-level antibodies co...

the aim of this study was to evaluate an enzyme - linked immunosorbent assay (elisa) for the detection of anti - helicobacter pylori (h. pylori) specific igg antibodies in specimens of oral fluid. all subjects over the age 18 years undergoing endoscopy for any reason were asked to participate in the study. two groups of 44 patients in each were selected as hp+ and hp-. at the same time, 5 milli...

background: timely diagnosis of leptospirosis is essential for early and effective treatment, for there are many differential diagnoses for it.. leptospiral researchers have an increasing interest in developing new serological methods with recombinant antigens to improve the leptospirosis diagnosis. several serological tests have been developed for the proper diagnosis of leptospirosis. objecti...

objectives: leptin is a hormone-like protein produced by the adipose tissue. it plays an important role in protection of host against inflammation and infection. some studies have reported changes in leptin levels in the gingival crevicular fluid (gcf), saliva and blood serum of patients with periodontal disease compared to healthy individuals. the aim of the present study was to compare the sa...

materials and methods in this descriptive study, 2988 stool samples of patients with acute gastroenteritis were collected from children’s hospitals of 5 main cities of iran. the samples were sent in frozen condition to pediatric infection research center in tehran and stored at -70°c. elisa test was performed for detection of rotavirus antigens. the mean age of study population was 1 to 5 years...

conclusions: it was concluded that the elisa is a suitable method for large scale screening of antibodies against aspergilloma. the easy techniques have shortened the required time of tests (approximately 1.30 hours for our elisa, as opposed to 2.30 hours with commercial elisa kit). this may be a substantial benefit for large numbers of samples that should be tested. materials and methods: an i...

background: nuts are one of the main consumed snacks worldwide and also have an important role among iranian's food habits. natural contamination of nuts with aflatoxin is unavoidable and causes a special challenge for nuts safety and quality. objectives: the purpose of this research was to study the aflatoxin contamination in commercially-available nuts (pistachio, walnut and peanut) in the ma...

b ackground : tuberculosis is a crucial health problem. establishing a rapid, reliable and still inexpensive diagnostic method for tuberculosis seems to be substantial in developing countries where tb has very high incidence rate . methods:   an indirect  enzyme-linked  immunosorbent  assay (elisa )   was established to detect serum antibodies against mycobacterium  tuberculosis. three kinds of...