Stutter products are a common artifact in the PCR amplification of frequently used genetic markers that contain mononucleotide simple sequence repeats. Despite the importance of accurate determination of nucleotide sequence and allele size, there has been little progress toward decreasing the formation of stutter products during PCR. In this study, we tested the effects of lowered extension tem...

The polymerase chain reaction (PCR) (2) has evolved into one of the most powerful and widely used tools in molecular biology. Apart from its application in basic research, the universal nature of the PCR technique has proven extremely useful in the field of medical microbiology for the diagnosis and surveillance of infectious diseases. Although the technique is distinguished by its outstanding ...

ABO is the most clinically important blood group system in transfusion and transplantation medicine. The popular ABO genotyping methods, such as the sequencing of exons 6 and 7 and sequence-specific primer (SSP)-PCR, often lead to ambiguous typing results. Long PCR-sequencing method was designed to analyze two regulatory regions (promoter and CBF/NF-Y enhancer regions) and all genomic sequences...

Background: COVID-19 was an unknown disease previously. This pandemic has affected almost all countries of the world and creating a deadly experience to all. It leads long term squeals among many people with consequent loss productivity quality life. entity is called COVID syndrome. The objectives present study find out sequels patients.
 Materials methods: observational where total 56 pat...

In this study we amplified virtually the entire genomes of hepatitis A virus (a member of the Picornaviridae family), hepatitis B virus (a member of the Hepadnaviridae family), and hepatitis C virus (a member of the Flaviviridae family) by using the recently described technique of long PCR. In order to do this, we first demonstrated, using the lambda phage, that long PCR can be made highly sens...