The boundary element technique is a practical problem-solving tool and useful in comparison to other currently popular alternatives. In this paper, a circular tunnel of a diameter 3.4 m is to be excavated through a clayey soil and surrounded by concrete lining of a thickness 0.2 m, so the clear internal diameter is 3.0 m. A plane strain analysis is adopted for such problem and the soil is assum...

A defined medium (medium MB) for Moraxella bovis was formulated. Nineteen strains grew well on medium MB. One strain was auxotrophic for asparagine, and another was auxotrophic for methionine. Strains of M. equi and M. lacunata also grew on medium MB. All strains had an absolute requirement for thiamine and were stimulated by or actually required the other growth factors in the medium.

Mycobacterium ulcerans, the etiologic agent of Buruli ulcer, has been detected on aquatic plants in endemic tropical regions. Here, we tested the effect of several tropical plant extracts on the growth of M. ulcerans and the closely related Mycobacterium marinum. M. ulcerans and M. marinum were inoculated on Middlebrook 7H11 medium with and without extracts from tropical aquatic plants, includi...

In an attempt to isolate multipotent stem cells from foreskin in a long-term culture, we encountered an interesting phenomenon which was the conversion of the fibroblast dominant condition to epithelial-like tissue formation. However, the basic mechanism(s) which may be involved in this conversion is not clear. This study was designed to evaluate the cells protein secretion activity and examine...

production of extracellular amylase was demonstrated under conditions of high salinity in aerobically cultivated culture of a newly isolated moderately halophilic gram-positive coccus, designated strain f in basal medium containing peptone from meat, yeast extract, nacl (7% w/v) and starch. biochemical and physiological characterization along with 16s rrna sequence analysis placed f in the genu...

Five pairs of rabbit corneas were stored for two weeks at 4 degrees C, one of each pair in K-Sol medium, and one in McCarey-/Kaufman (M-K) medium. After transplantation all penetrating keratoplasty grafts became clear and thin. Endothelial cell loss was significantly less in the K-Sol stored corneas. Another five pairs of corneas were stored for two weeks in K-Sol or three days in M-K medium. A...

Phosphorus solubilizing microorganisms are capable of transforming insoluble phosphorus (P) forms to soluble and bioavailable P. The aim of the present study was to isolate P solubilizing bacteria from forest soils around the rock phosphate (RP) mine of Koh-e-SepidLar in Kohgiluyeh and Boyer Ahmad province and evaluate their ability in Sperber culture medium and wheat straw medium in the presen...